Two VH5 family genes expressed by human peripheral B cells display differential mutational frequencies in the VH region

Yong Chong, Hideyuki Ikematsu, Masayuki Murata, Kouzaburo Yamaji, Shigeki Nabeshima, Seizaburo Kashiwagi, Jun Hayashi

Research output: Contribution to journalArticlepeer-review

Abstract

The heavy chain variable segment gene (VH)5 family, one of the seven immunoglobulin (Ig) VH families, contains two functional genes, VH251 and VH32. To investigate functional differences between these VH5 family genes, VH segments expressed by human peripheral B cells were sequenced and analyzed. One hundred fifty-three sequences with unique VHDJH recombinations were obtained from 17 adults. The mutational frequency of VH32 derived sequences (6.4%) was higher than that of VH251 derived sequences (4.4%), resulting in a significant difference (P<0.01). Significant differences in mutational frequencies between VH251 and VH32 derived sequences were observed in CDRs and FRs. No significant differences were found in CDR3 length distribution, D segment usage, or JH segment usage between VH251 and VH32 derived sequences. These results suggest that mutational frequency is affected, in part, by VH gene structure. The difference may occur after recombinational events in B cell development.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalMolecular Immunology
Volume39
Issue number1-2
DOIs
Publication statusPublished - Sep 15 2002

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

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