Tyk2 is essential for IFN-α-induced gene expression in mast cells

Yumiko Mori, Koichi Hirose, Kotaro Suzuki, Hiroshi Nakajima, Yohei Seto, Kei Ikeda, Kazuya Shimoda, Kei Ichi Nakayama, Yasushi Saito, Itsuo Iwamoto

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9 Citations (Scopus)

Abstract

Mast cells are recognized not only as the major effector cells of type I hypersensitivity reactions but also as an important player of innate immune response against bacterial infection. Type I IFNs are also involved in the response against bacterial infection. However, the role of type I IFNs and their associated Janus kinase Tyk2 in mast cell functions remains to be determined. In this study, we addressed this issue using Tyk2-deficient (Tyk2-/-) bone marrow-derived mast cells (BMMCs). When BMMCs from wild-type (WT) mice were stimulated with IFN-α, they expressed mRNA for IFN-γ-inducible protein 10 (IP-10) and monocyte chemoattractant protein-5 (MCP-5). Interestingly, IFN-α-induced expression of IP-10 and MCP-5 was severely decreased in Tyk2-/- BMMCs. In addition, IFN-α-induced Stat1 phosphorylation was decreased in Tyk2-/- BMMCs. On the other hand, IFN-α-induced Stat1 phosphorylation and IP-10 and MCP-5 expression were normal in Tyk2-/- fibroblasts. These results indicate that IFN-α induces the expression of TNF-α and the chemokines IP-10 and MCP-5 in mast cells and that Tyk2 plays a nonredundant role in IFN-α signaling in mast cells.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalInternational Archives of Allergy and Immunology
Volume134
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 15 2004

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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    Mori, Y., Hirose, K., Suzuki, K., Nakajima, H., Seto, Y., Ikeda, K., Shimoda, K., Nakayama, K. I., Saito, Y., & Iwamoto, I. (2004). Tyk2 is essential for IFN-α-induced gene expression in mast cells. International Archives of Allergy and Immunology, 134(SUPPL. 1), 25-29. https://doi.org/10.1159/000077789