Tyk2 is essential for IFN-α-induced gene expression in mast cells

Yumiko Mori, Koichi Hirose, Kotaro Suzuki, Hiroshi Nakajima, Yohei Seto, Kei Ikeda, Kazuya Shimoda, Kei Ichi Nakayama, Yasushi Saito, Itsuo Iwamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mast cells are recognized not only as the major effector cells of type I hypersensitivity reactions but also as an important player of innate immune response against bacterial infection. Type I IFNs are also involved in the response against bacterial infection. However, the role of type I IFNs and their associated Janus kinase Tyk2 in mast cell functions remains to be determined. In this study, we addressed this issue using Tyk2-deficient (Tyk2-/-) bone marrow-derived mast cells (BMMCs). When BMMCs from wild-type (WT) mice were stimulated with IFN-α, they expressed mRNA for IFN-γ-inducible protein 10 (IP-10) and monocyte chemoattractant protein-5 (MCP-5). Interestingly, IFN-α-induced expression of IP-10 and MCP-5 was severely decreased in Tyk2-/- BMMCs. In addition, IFN-α-induced Stat1 phosphorylation was decreased in Tyk2-/- BMMCs. On the other hand, IFN-α-induced Stat1 phosphorylation and IP-10 and MCP-5 expression were normal in Tyk2-/- fibroblasts. These results indicate that IFN-α induces the expression of TNF-α and the chemokines IP-10 and MCP-5 in mast cells and that Tyk2 plays a nonredundant role in IFN-α signaling in mast cells.

Original languageEnglish
Pages (from-to)25-29
Number of pages5
JournalInternational Archives of Allergy and Immunology
Volume134
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 15 2004

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Mast Cells
Monocyte Chemoattractant Proteins
Gene Expression
Bone Marrow
Bacterial Infections
Proteins
Phosphorylation
Janus Kinases
Immediate Hypersensitivity
Chemokines
Innate Immunity
Fibroblasts
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Mori, Y., Hirose, K., Suzuki, K., Nakajima, H., Seto, Y., Ikeda, K., ... Iwamoto, I. (2004). Tyk2 is essential for IFN-α-induced gene expression in mast cells. International Archives of Allergy and Immunology, 134(SUPPL. 1), 25-29. https://doi.org/10.1159/000077789

Tyk2 is essential for IFN-α-induced gene expression in mast cells. / Mori, Yumiko; Hirose, Koichi; Suzuki, Kotaro; Nakajima, Hiroshi; Seto, Yohei; Ikeda, Kei; Shimoda, Kazuya; Nakayama, Kei Ichi; Saito, Yasushi; Iwamoto, Itsuo.

In: International Archives of Allergy and Immunology, Vol. 134, No. SUPPL. 1, 15.06.2004, p. 25-29.

Research output: Contribution to journalArticle

Mori, Y, Hirose, K, Suzuki, K, Nakajima, H, Seto, Y, Ikeda, K, Shimoda, K, Nakayama, KI, Saito, Y & Iwamoto, I 2004, 'Tyk2 is essential for IFN-α-induced gene expression in mast cells', International Archives of Allergy and Immunology, vol. 134, no. SUPPL. 1, pp. 25-29. https://doi.org/10.1159/000077789
Mori Y, Hirose K, Suzuki K, Nakajima H, Seto Y, Ikeda K et al. Tyk2 is essential for IFN-α-induced gene expression in mast cells. International Archives of Allergy and Immunology. 2004 Jun 15;134(SUPPL. 1):25-29. https://doi.org/10.1159/000077789
Mori, Yumiko ; Hirose, Koichi ; Suzuki, Kotaro ; Nakajima, Hiroshi ; Seto, Yohei ; Ikeda, Kei ; Shimoda, Kazuya ; Nakayama, Kei Ichi ; Saito, Yasushi ; Iwamoto, Itsuo. / Tyk2 is essential for IFN-α-induced gene expression in mast cells. In: International Archives of Allergy and Immunology. 2004 ; Vol. 134, No. SUPPL. 1. pp. 25-29.
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