Typing Method for the QUB11a Locus of Mycobacterium tuberculosis: IS 6110 Insertions and Tandem Repeat Analysis

Eriko Maeda-Mitani, Koichi Murakami, Akira Oishi, Yoshiki Etoh, Nobuyuki Sera, Shuji Fujimoto

Research output: Contribution to journalArticlepeer-review

Abstract

QUB11a is used as a locus for variable number of tandem repeats (VNTR) analysis of Mycobacterium tuberculosis Beijing lineage. However, amplification of QUB11a occasionally produces large fragments (>1,400 bp) that are not easily measured by capillary electrophoresis because of a lack of the typical stutter peak patterns that are used for counting repeat numbers. IS6110 insertion may complicate VNTR analysis of large QUB11a fragments in M. tuberculosis. We established a method for determining both tandem repeat numbers and IS6110 insertion in the QUB11a locus of M. tuberculosis using capillary electrophoresis analysis and BsmBI digestion. All 29 large QUB11a fragments (>1,200 bp) investigated contained IS6110 insertions and varied in the number of repeats (18 patterns) and location of IS6110 insertions. This method allows VNTR analysis with high discrimination.

Original languageEnglish
Article number5216530
JournalBioMed Research International
Volume2016
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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