Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting

Jacques G. Mainil, M. Bardiau, T. Ooka, Yoshitoshi Ogura, K. Murase, Y. Etoh, S. Ichihara, K. Horikawa, G. Buvens, D. Piérard, T. Itoh, Tetsuya Hayashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. Methods and Results: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. Conclusions: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. Significance and Impact of the Study: The IS621 printing method represents a rapid (24h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks.

Original languageEnglish
Pages (from-to)773-786
Number of pages14
JournalJournal of Applied Microbiology
Volume111
Issue number3
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

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Enterohemorrhagic Escherichia coli
Enteropathogenic Escherichia coli
Multiplex Polymerase Chain Reaction
Pulsed Field Gel Electrophoresis
Dermatoglyphics
Disease Outbreaks
Chromosomes, Human, Pair 12
Printing
Belgium
Japan
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting. / Mainil, Jacques G.; Bardiau, M.; Ooka, T.; Ogura, Yoshitoshi; Murase, K.; Etoh, Y.; Ichihara, S.; Horikawa, K.; Buvens, G.; Piérard, D.; Itoh, T.; Hayashi, Tetsuya.

In: Journal of Applied Microbiology, Vol. 111, No. 3, 01.09.2011, p. 773-786.

Research output: Contribution to journalArticle

Mainil, Jacques G. ; Bardiau, M. ; Ooka, T. ; Ogura, Yoshitoshi ; Murase, K. ; Etoh, Y. ; Ichihara, S. ; Horikawa, K. ; Buvens, G. ; Piérard, D. ; Itoh, T. ; Hayashi, Tetsuya. / Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting. In: Journal of Applied Microbiology. 2011 ; Vol. 111, No. 3. pp. 773-786.
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