Tyrosine kinase 2 interacts with and phosphorylates receptor for activated C kinase-1, a WD motif-containing protein

Takashi Haro, Kazuya Shimoda, Haruko Kakumitsu, Kenjiro Kamezaki, Akihiko Numata, Fumihiko Ishikawa, Yuichi Sekine, Ryuta Muromoto, Tadashi Matsuda, Mine Harada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Receptor for activated C kinase (Rack)-1 is a protein kinase C-interacting protein, and contains a WD repeat but has no enzymatic activity. In addition to protein kinase C, Rack-1 also binds to Src, phospholipase Cγ, and ras-GTPase-activating proteins. Thus, Rack-1 is thought to function as a scaffold protein that recruits specific signaling elements. In a cytokine signaling cascade, Rack-1 has been reported to interact with the IFN-αβ receptor and Stat1. In addition, we show here that Rack-1 associates with a member of Jak, tyrosine kinase 2 (Tyk2). Rack-1 interacts weakly with the kinase domain and interacts strongly with the pseudokinase domain of Tyk2. Rack-1 associates with Tyk2 via two regions, one in the N terminus and one in the middle portion (aa 138-203) of Rack-1. Jak activation causes the phosphorylation of tyrosine 194 on Rack-1. After phosphorylation, Rack-1 is translocated toward the perinuclear region. In addition to functioning as a scaffolding protein, these results raise the possibility that Rack-1 functions as a signaling molecule in cytokine signaling cascades.

Original languageEnglish
Pages (from-to)1151-1157
Number of pages7
JournalJournal of Immunology
Volume173
Issue number2
DOIs
Publication statusPublished - Jul 15 2004

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TYK2 Kinase
Amino Acid Motifs
receptors for activated C kinase
ras GTPase-Activating Proteins
Phosphorylation
Cytokines
Proteins
Type C Phospholipases
Protein Kinase C

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Tyrosine kinase 2 interacts with and phosphorylates receptor for activated C kinase-1, a WD motif-containing protein. / Haro, Takashi; Shimoda, Kazuya; Kakumitsu, Haruko; Kamezaki, Kenjiro; Numata, Akihiko; Ishikawa, Fumihiko; Sekine, Yuichi; Muromoto, Ryuta; Matsuda, Tadashi; Harada, Mine.

In: Journal of Immunology, Vol. 173, No. 2, 15.07.2004, p. 1151-1157.

Research output: Contribution to journalArticle

Haro, T, Shimoda, K, Kakumitsu, H, Kamezaki, K, Numata, A, Ishikawa, F, Sekine, Y, Muromoto, R, Matsuda, T & Harada, M 2004, 'Tyrosine kinase 2 interacts with and phosphorylates receptor for activated C kinase-1, a WD motif-containing protein', Journal of Immunology, vol. 173, no. 2, pp. 1151-1157. https://doi.org/10.4049/jimmunol.173.2.1151
Haro, Takashi ; Shimoda, Kazuya ; Kakumitsu, Haruko ; Kamezaki, Kenjiro ; Numata, Akihiko ; Ishikawa, Fumihiko ; Sekine, Yuichi ; Muromoto, Ryuta ; Matsuda, Tadashi ; Harada, Mine. / Tyrosine kinase 2 interacts with and phosphorylates receptor for activated C kinase-1, a WD motif-containing protein. In: Journal of Immunology. 2004 ; Vol. 173, No. 2. pp. 1151-1157.
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