Tyrosine phosphorylation of G protein α subunits by pp60(c-src)

W. P. Hausdorff, J. A. Pitcher, D. K. Luttrell, M. E. Linder, H. Kurose, S. J. Parsons, M. G. Caron, R. J. Lefkowitz

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Abstract

A number of lines of evidence suggest that cross-talk exists between the cellular signal transduction pathways involving tyrosine phosphorylation catalyzed by members of the pp60(c-src) kinase family and those mediated by guanine nucleotide regulatory proteins (G proteins). In this study, we explore the possibility that direct interactions between pp60(c-src) and G proteins may occur with functional consequences. Preparations of pp60(c-src) isolated by immunoprecipitation phosphorylate on tyrosine residues the purified G-protein α subunits (Gα) of several heterotrimeric G proteins. Phosphorylation is highly dependent on G-protein conformation, and Gα(GDP) uncomplexed by βγ subunits appears to be the preferred substrate. In functional studies, phosphorylation of stimulatory Gα (Gα(s)) modestly increases the rate of binding of guanosine 5'-[γ-[35S]thio]triphosphate to G(s) as well as the receptor-stimulated steady-state rate of GTP hydrolysis by G(s). Heterotrimeric G proteins may represent a previously unappreciated class of potential substrates for pp60(c-src).

Original languageEnglish
Pages (from-to)5720-5724
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number13
DOIs
Publication statusPublished - Jul 20 1992

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Proto-Oncogene Proteins pp60(c-src)
Protein Subunits
GTP-Binding Proteins
Tyrosine
Phosphorylation
Protein Conformation
Guanosine
Guanosine Triphosphate
Immunoprecipitation
Signal Transduction
Hydrolysis

All Science Journal Classification (ASJC) codes

  • General

Cite this

Hausdorff, W. P., Pitcher, J. A., Luttrell, D. K., Linder, M. E., Kurose, H., Parsons, S. J., ... Lefkowitz, R. J. (1992). Tyrosine phosphorylation of G protein α subunits by pp60(c-src). Proceedings of the National Academy of Sciences of the United States of America, 89(13), 5720-5724. https://doi.org/10.1073/pnas.89.13.5720

Tyrosine phosphorylation of G protein α subunits by pp60(c-src). / Hausdorff, W. P.; Pitcher, J. A.; Luttrell, D. K.; Linder, M. E.; Kurose, H.; Parsons, S. J.; Caron, M. G.; Lefkowitz, R. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 13, 20.07.1992, p. 5720-5724.

Research output: Contribution to journalArticle

Hausdorff, WP, Pitcher, JA, Luttrell, DK, Linder, ME, Kurose, H, Parsons, SJ, Caron, MG & Lefkowitz, RJ 1992, 'Tyrosine phosphorylation of G protein α subunits by pp60(c-src)', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 13, pp. 5720-5724. https://doi.org/10.1073/pnas.89.13.5720
Hausdorff, W. P. ; Pitcher, J. A. ; Luttrell, D. K. ; Linder, M. E. ; Kurose, H. ; Parsons, S. J. ; Caron, M. G. ; Lefkowitz, R. J. / Tyrosine phosphorylation of G protein α subunits by pp60(c-src). In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 13. pp. 5720-5724.
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