U-box proteins as a new family of ubiquitin ligases

Shigetsugu Hatakeyama, Kei Ichi I. Nakayama

Research output: Contribution to journalReview article

149 Citations (Scopus)

Abstract

Ubiquitin-protein ligases (E3s) determine the substrate specificity of ubiquitylation and, until recently, had been classified into two families, the HECT and RING-finger families. The U-box is a domain of ∼70 amino acids that is present in proteins from yeast to humans. The prototype U-box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and an E3 to catalyze the formation of a ubiquitin chain on artificial substrates. We recently showed that mammalian U-box proteins, in conjunction with an E1 and an E2, mediate polyubiquitylation in the absence of a HECT type or RING-finger type E3. U-box proteins have thus been defined as a third family of E3s. We here review recent progress in the characterization of U-box proteins and of their role in the quality control system that underlies the cellular stress response to the intracellular accumulation of abnormal proteins.

Original languageEnglish
Pages (from-to)635-645
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number4
DOIs
Publication statusPublished - Mar 21 2003

Fingerprint

Ligases
Ubiquitin
Fungal Proteins
Fingers
Proteins
Ubiquitin-Activating Enzymes
Ubiquitin-Conjugating Enzymes
Ubiquitin-Protein Ligases
Ubiquitination
Substrates
Substrate Specificity
Quality Control
Quality control
Control systems
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

U-box proteins as a new family of ubiquitin ligases. / Hatakeyama, Shigetsugu; Nakayama, Kei Ichi I.

In: Biochemical and Biophysical Research Communications, Vol. 302, No. 4, 21.03.2003, p. 635-645.

Research output: Contribution to journalReview article

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