Ubiquinone biosynthesis by mitochondria, sonicated mitochondria and mitoplasts of rat liver

Dongchon Kang, Toshiyuki Fujiwara, Koichiro Takeshige

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ubiquinone was biosynthesized when rat liver mitochondria were incubated with S-adenosyl-L-methionine, solanesyl diphosphate, and [U-14C]p-hydroxybenzoate. The intermediates of ubiquinone biosynthesis but not ubiquinone were accumulated in mitochondria incubated without S-adenosyl-L-methionine and the accumulated intermediates were converted to ubiquinone by the addition of the methyl group donor and an excess of cold p-hydroxybenzoate. No solaneylated compounds except nonaprenyl p-hydroxybenzoate were found in sonicated mitochondria, while the biosynthesis of ubiquinone was observed in the sonicated preparation of mitochondria in which the intermediates accumulated. The results indicate that the initial decarboxylation reaction is completely abolished and the subsequent reactions of hydroxylation and methylation are not completely inhibited by the sonication treatment and therefore the decarboxylation reaction is the next step after nonaprenylation of p-hydroxybenzoate. Mitoplasts could biosynthesize ubiquinone with activity comparable to that of intact mitochondria, suggesting that components of the outer membrane and the intermembranous space of mitochondria are not involved in ubiquinone biosynthesis.

Original languageEnglish
Pages (from-to)371-375
Number of pages5
JournalJournal of Biochemistry
Volume111
Issue number3
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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Mitochondria
Ubiquinone
Biosynthesis
Liver
Rats
S-Adenosylmethionine
Decarboxylation
Hydroxylation
Sonication
Methylation
Liver Mitochondrion
Membranes
4-hydroxybenzoic acid

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Ubiquinone biosynthesis by mitochondria, sonicated mitochondria and mitoplasts of rat liver. / Kang, Dongchon; Fujiwara, Toshiyuki; Takeshige, Koichiro.

In: Journal of Biochemistry, Vol. 111, No. 3, 01.01.1992, p. 371-375.

Research output: Contribution to journalArticle

Kang, Dongchon ; Fujiwara, Toshiyuki ; Takeshige, Koichiro. / Ubiquinone biosynthesis by mitochondria, sonicated mitochondria and mitoplasts of rat liver. In: Journal of Biochemistry. 1992 ; Vol. 111, No. 3. pp. 371-375.
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