Ubiquitin chains in the Dsk2 UBL domain mediate Dsk2 stability and protein degradation in yeast

Takeshi Sekiguchi, Toru Sasaki, Minoru Funakoshi, Takashi Ishii, Yoh hei Saitoh, Shu ichi Kaneko, Hideki Kobayashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ubiquitin-like (UBL)-ubiquitin-associated (UBA) proteins, including Dsk2 and Rad23, act as delivery factors that target polyubiquitinated substrates to the proteasome. We report here that the Dsk2 UBL domain is ubiquitinated in yeast cells and that Dsk2 ubiquitination of the UBL domain is involved in Dsk2 stability, depending on the Dsk2 UBA domain. Also, Dsk2 lacking ubiquitin chains impaired ubiquitin-dependent protein degradation and decreased the interaction of Dsk2 with polyubiquitinated proteins in cells. Moreover, Dsk2 ubiquitination affected ability to restore the temperature-sensitive growth defect of dsk2Δ. These results indicate that ubiquitination in the UBL domain of Dsk2 has in vivo functions in the ubiquitin-proteasome pathway in yeast.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume411
Issue number3
DOIs
Publication statusPublished - Aug 5 2011

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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