Ubiquitin fusion proteins are overexpressed in colon cancer but not in gastric cancer

Graham F. Barnard, Masaki Mori, Raymond J. Staniunas, Nasim A. Begum, Shideng Bao, Mark Puder, Joseph Cobb, Kent L. Redman, Glenn D. Steele, Lan Bo Chen

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A cDNA clone (AF3) encoding the ubiquitin A gene 52 amino acid extension fusion protein (UbA52) was isolated from a subtracted cDNA library of human colorectal carcinoma minus adjacent normal mucosa. In Northern hybridization the mRNA signal for UbA52 was greater in surgical samples of colonic carcinoma (T) than in paired adjacent normal (N) tissues in 24 of 29 cases (T/N = 3.4 ± 0.5, P < 0.01). An oligonucleotide probe specific for only the 52 amino acid extension confirmed the overexpression of UbA52. In contrast, there was no overexpression of UbA52 mRNA in gastric cancer samples (n = 7, T/N = 1.0 ± 0.3). The mRNA of several ribosomal proteins, and of another ubiquitin A gene fusion protein, UbA80, with an 80 amino acid extension of ribosomal protein S27a, have been reported to be over-expressed in colon cancer, but not as yet at the protein level. Using rabbit antisera to the ribosomal protein component S27a we demonstrate over-expression of S27a at the protein level in colonic (n = 5), but not gastric (n = 6) carcinomas. Therefore it is likely that both UbA80 and UbA52 are overexpressed in colon cancer, but not in gastric cancer.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalBBA - Molecular Basis of Disease
Volume1272
Issue number3
DOIs
Publication statusPublished - Dec 12 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology

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