Ubiquitin mediates the physical and functional interaction between human DNA polymerases η and ι

Justyna McIntyre, Antonio E. Vidal, Mary P. McLenigan, Martha G. Bomar, Elena Curti, John P. McDonald, Brian S. Plosky, Eiji Ohashi, Roger Woodgate

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19 Citations (Scopus)

Abstract

Human DNA polymerases η and ι are best characterized for their ability to facilitate translesion DNA synthesis (TLS). Both polymerases (pols) co-localize in 'replication factories' in vivo after cells are exposed to ultraviolet light and this co-localization is mediated through a physical interaction between the two TLS pols. We have mapped the polη-ι interacting region to their respective ubiquitin-binding domains (UBZ in polη and UBM1 and UBM2 in polι), and demonstrate that ubiquitination of either TLS polymerase is a prerequisite for their physical and functional interaction. Importantly, while monoubiquitination of polη precludes its ability to interact with proliferating cell nuclear antigen (PCNA), it enhances its interaction with polι. Furthermore, a polι-ubiquitin chimera interacts avidly with both polη and PCNA. Thus, the ubiquitination status of polη, or polι plays a key regulatory function in controlling the protein partners with which each polymerase interacts, and in doing so, determines the efficiency of targeting the respective polymerase to stalled replication forks where they facilitate TLS.

Original languageEnglish
Pages (from-to)1649-1660
Number of pages12
JournalNucleic acids research
Volume41
Issue number3
DOIs
Publication statusPublished - Feb 2013

All Science Journal Classification (ASJC) codes

  • Genetics

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    McIntyre, J., Vidal, A. E., McLenigan, M. P., Bomar, M. G., Curti, E., McDonald, J. P., Plosky, B. S., Ohashi, E., & Woodgate, R. (2013). Ubiquitin mediates the physical and functional interaction between human DNA polymerases η and ι. Nucleic acids research, 41(3), 1649-1660. https://doi.org/10.1093/nar/gks1277