Ubiquitous expression of myostatin in chicken embryonic tissues: Its high expression in testis and ovary

Kaiyu Kubota, Fuminori Sato, Shinya Aramaki, Tomoki Soh, Nobuhiko Yamauchi, Masa aki Hattori

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The skeletal muscle of mammals is known to express myostatin (GDF-8) that acts as a potent negative regulator of skeletal muscle growth. However, the function of GDF-8 is not limited to skeletal muscle, because of its ubiquitous expression in fish. Here we investigated whether GDF-8 is expressed in various tissues including gonads during chicken embryogenesis. As revealed by RT-PCR and Western blotting, the transcript and protein for GDF-8 were detected in brain, eye, gizzard, muscle, heart, small gut, large gut, mesonephroi, testis and ovary of chicken embryos at E12, but not in liver. GDF-8 was constitutively expressed in testis and ovary as well as muscle at E6-E21, as demonstrated by in situ hybridization on section and whole-mount. Some cell population in testis, but not identified, highly expressed GDF-8. On the other hand, the medulla and germinal epithelium of ovary highly expressed it. Collectively, these results indicate that GDF-8 is ubiquitously expressed in various tissues of chicken embryos including testis and ovary through the stage of embryogenesis.

Original languageEnglish
Pages (from-to)550-555
Number of pages6
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume148
Issue number3
DOIs
Publication statusPublished - Nov 1 2007

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Myostatin
Testis
Ovary
Chickens
Tissue
Muscle
Skeletal Muscle
Embryonic Development
Embryonic Structures
Mesonephros
Avian Gizzard
Mammals
Gonads
Liver
Fish
In Situ Hybridization
Brain
Myocardium
Fishes
Epithelium

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

Ubiquitous expression of myostatin in chicken embryonic tissues : Its high expression in testis and ovary. / Kubota, Kaiyu; Sato, Fuminori; Aramaki, Shinya; Soh, Tomoki; Yamauchi, Nobuhiko; Hattori, Masa aki.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 148, No. 3, 01.11.2007, p. 550-555.

Research output: Contribution to journalArticle

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AU - Hattori, Masa aki

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