TY - JOUR
T1 - Ubiquitous expression of myostatin in chicken embryonic tissues
T2 - Its high expression in testis and ovary
AU - Kubota, Kaiyu
AU - Sato, Fuminori
AU - Aramaki, Shinya
AU - Soh, Tomoki
AU - Yamauchi, Nobuhiko
AU - Hattori, Masa aki
N1 - Funding Information:
This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Sciences (to M-A. H).
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2007/11
Y1 - 2007/11
N2 - The skeletal muscle of mammals is known to express myostatin (GDF-8) that acts as a potent negative regulator of skeletal muscle growth. However, the function of GDF-8 is not limited to skeletal muscle, because of its ubiquitous expression in fish. Here we investigated whether GDF-8 is expressed in various tissues including gonads during chicken embryogenesis. As revealed by RT-PCR and Western blotting, the transcript and protein for GDF-8 were detected in brain, eye, gizzard, muscle, heart, small gut, large gut, mesonephroi, testis and ovary of chicken embryos at E12, but not in liver. GDF-8 was constitutively expressed in testis and ovary as well as muscle at E6-E21, as demonstrated by in situ hybridization on section and whole-mount. Some cell population in testis, but not identified, highly expressed GDF-8. On the other hand, the medulla and germinal epithelium of ovary highly expressed it. Collectively, these results indicate that GDF-8 is ubiquitously expressed in various tissues of chicken embryos including testis and ovary through the stage of embryogenesis.
AB - The skeletal muscle of mammals is known to express myostatin (GDF-8) that acts as a potent negative regulator of skeletal muscle growth. However, the function of GDF-8 is not limited to skeletal muscle, because of its ubiquitous expression in fish. Here we investigated whether GDF-8 is expressed in various tissues including gonads during chicken embryogenesis. As revealed by RT-PCR and Western blotting, the transcript and protein for GDF-8 were detected in brain, eye, gizzard, muscle, heart, small gut, large gut, mesonephroi, testis and ovary of chicken embryos at E12, but not in liver. GDF-8 was constitutively expressed in testis and ovary as well as muscle at E6-E21, as demonstrated by in situ hybridization on section and whole-mount. Some cell population in testis, but not identified, highly expressed GDF-8. On the other hand, the medulla and germinal epithelium of ovary highly expressed it. Collectively, these results indicate that GDF-8 is ubiquitously expressed in various tissues of chicken embryos including testis and ovary through the stage of embryogenesis.
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U2 - 10.1016/j.cbpa.2007.07.004
DO - 10.1016/j.cbpa.2007.07.004
M3 - Article
C2 - 17707668
AN - SCOPUS:34748880039
SN - 1095-6433
VL - 148
SP - 550
EP - 555
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
IS - 3
ER -