Abstract
Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16 INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 a denom a spe c i m en s, a na lyz ed t he relat ionsh ip b et we en U H R F1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8%) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5%). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (P=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (P=0.051). UHRF1 expression was significantly associated with E2F-1 expression (P<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.
| Original language | English |
|---|---|
| Pages (from-to) | 1997-2002 |
| Number of pages | 6 |
| Journal | Oncology reports |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 1 2012 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. / Kofunato, Yasuhide; Kumamoto, Kensuke; Saitou, Katsuharu; Hayase, Suguru; Okayama, Hirokazu; Miyamoto, Kotaro; Sato, Yu; Katakura, Kyoko; Nakamura, Izumi; Ohki, Shinji; Koyama, Yoshihisa; Unoki, Motoko; Takenoshita, Seiichi.
In: Oncology reports, Vol. 28, No. 6, 01.12.2012, p. 1997-2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer
AU - Kofunato, Yasuhide
AU - Kumamoto, Kensuke
AU - Saitou, Katsuharu
AU - Hayase, Suguru
AU - Okayama, Hirokazu
AU - Miyamoto, Kotaro
AU - Sato, Yu
AU - Katakura, Kyoko
AU - Nakamura, Izumi
AU - Ohki, Shinji
AU - Koyama, Yoshihisa
AU - Unoki, Motoko
AU - Takenoshita, Seiichi
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16 INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 a denom a spe c i m en s, a na lyz ed t he relat ionsh ip b et we en U H R F1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8%) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5%). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (P=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (P=0.051). UHRF1 expression was significantly associated with E2F-1 expression (P<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.
AB - Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16 INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 a denom a spe c i m en s, a na lyz ed t he relat ionsh ip b et we en U H R F1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8%) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5%). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (P=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (P=0.051). UHRF1 expression was significantly associated with E2F-1 expression (P<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.
UR - http://www.scopus.com/inward/record.url?scp=84868133938&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868133938&partnerID=8YFLogxK
U2 - 10.3892/or.2012.2064
DO - 10.3892/or.2012.2064
M3 - Article
C2 - 23023523
AN - SCOPUS:84868133938
VL - 28
SP - 1997
EP - 2002
JO - Oncology Reports
JF - Oncology Reports
SN - 1021-335X
IS - 6
ER -