UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer

Yasuhide Kofunato, Kensuke Kumamoto, Katsuharu Saitou, Suguru Hayase, Hirokazu Okayama, Kotaro Miyamoto, Yu Sato, Kyoko Katakura, Izumi Nakamura, Shinji Ohki, Yoshihisa Koyama, Motoko Unoki, Seiichi Takenoshita

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16 INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 a denom a spe c i m en s, a na lyz ed t he relat ionsh ip b et we en U H R F1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8%) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5%). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (P=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (P=0.051). UHRF1 expression was significantly associated with E2F-1 expression (P<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.

    Original languageEnglish
    Pages (from-to)1997-2002
    Number of pages6
    JournalOncology reports
    Volume28
    Issue number6
    DOIs
    Publication statusPublished - Dec 1 2012

    Fingerprint

    Colorectal Neoplasms
    Cell Proliferation
    Colonic Neoplasms
    Neoplasms
    Tumor Suppressor Protein p14ARF
    Cyclin-Dependent Kinase Inhibitor p16
    Staining and Labeling
    Cell Line
    Methyltransferases
    Growth
    Ubiquitin
    Tumor Suppressor Genes
    Cell Nucleus
    Adenoma
    Small Interfering RNA
    Fingers
    Carcinogenesis
    Mucous Membrane
    DNA

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cite this

    Kofunato, Y., Kumamoto, K., Saitou, K., Hayase, S., Okayama, H., Miyamoto, K., ... Takenoshita, S. (2012). UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. Oncology reports, 28(6), 1997-2002. https://doi.org/10.3892/or.2012.2064

    UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. / Kofunato, Yasuhide; Kumamoto, Kensuke; Saitou, Katsuharu; Hayase, Suguru; Okayama, Hirokazu; Miyamoto, Kotaro; Sato, Yu; Katakura, Kyoko; Nakamura, Izumi; Ohki, Shinji; Koyama, Yoshihisa; Unoki, Motoko; Takenoshita, Seiichi.

    In: Oncology reports, Vol. 28, No. 6, 01.12.2012, p. 1997-2002.

    Research output: Contribution to journalArticle

    Kofunato, Y, Kumamoto, K, Saitou, K, Hayase, S, Okayama, H, Miyamoto, K, Sato, Y, Katakura, K, Nakamura, I, Ohki, S, Koyama, Y, Unoki, M & Takenoshita, S 2012, 'UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer', Oncology reports, vol. 28, no. 6, pp. 1997-2002. https://doi.org/10.3892/or.2012.2064
    Kofunato Y, Kumamoto K, Saitou K, Hayase S, Okayama H, Miyamoto K et al. UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. Oncology reports. 2012 Dec 1;28(6):1997-2002. https://doi.org/10.3892/or.2012.2064
    Kofunato, Yasuhide ; Kumamoto, Kensuke ; Saitou, Katsuharu ; Hayase, Suguru ; Okayama, Hirokazu ; Miyamoto, Kotaro ; Sato, Yu ; Katakura, Kyoko ; Nakamura, Izumi ; Ohki, Shinji ; Koyama, Yoshihisa ; Unoki, Motoko ; Takenoshita, Seiichi. / UHRF1 expression is upregulated and associated with cellular proliferation in colorectal cancer. In: Oncology reports. 2012 ; Vol. 28, No. 6. pp. 1997-2002.
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    abstract = "Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of a number of tumor-suppressor genes, including p16 INK4A and p14ARF, by forming complexes with DNA methyltransferases and HDAC1, resulting in the induction of carcinogenesis. Altered UHRF1 expression has been demonstrated in various types of cancers. Previous reports indicate that UHRF1 expression is regulated by E2F-1 expression. We investigated UHRF1 expression using immunohistochemical staining in 231 colorectal cancer and 40 a denom a spe c i m en s, a na lyz ed t he relat ionsh ip b et we en U H R F1 expression and clinicopathological findings and the association between UHRF1 and E2F-1 expression. To better understand the biological function of UHRF1 in colorectal cancer, knockdown of UHRF1 expression was performed using siRNA methods. High UHRF1 expression was observed in 152 of 231 (65.8{\%}) colorectal cancer patients, and was detected in 35 of 40 adenoma specimens samples (87.5{\%}). UHRF1 staining was detected in the nucleus of cancer cells, while it was not detected in colonic normal mucosa. High UHRF1 expression was significantly observed in right compared with left hemicolon cancer (P=0.008). Moreover, high UHRF1 expression tended to be associated with depth of invasion (P=0.051). UHRF1 expression was significantly associated with E2F-1 expression (P<0.0001). Knockdown of UHRF1 expression suppressed cellular growth in colon cancer cell lines, HCT116 and SW620. In conclusion, we demonstrated that UHRF1 expression was upregulated in approximately two-thirds of colorectal cancer specimens and was particularly expressed in right compared with left hemicolon cancer. Moreover, knockdown of UHRF1 expression induced growth inhibition in colon cancer cell lines. UHRF1 may be involved in cellular proliferation and molecular pathogenesis of colorectal cancer in the right hemicolon.",
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    AU - Kofunato, Yasuhide

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    AU - Saitou, Katsuharu

    AU - Hayase, Suguru

    AU - Okayama, Hirokazu

    AU - Miyamoto, Kotaro

    AU - Sato, Yu

    AU - Katakura, Kyoko

    AU - Nakamura, Izumi

    AU - Ohki, Shinji

    AU - Koyama, Yoshihisa

    AU - Unoki, Motoko

    AU - Takenoshita, Seiichi

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