Ultrafast and wide range analysis of DNA molecules using rigid network structure of solid nanowires

Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Annop Klamchuen, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Analyzing sizes of DNA via electrophoresis using a gel has played an important role in the recent, rapid progress of biology and biotechnology. Although analyzing DNA over a wide range of sizes in a short time is desired, no existing electrophoresis methods have been able to fully satisfy these two requirements. Here we propose a novel method using a rigid 3D network structure composed of solid nanowires within a microchannel. This rigid network structure enables analysis of DNA under applied DC electric fields for a large DNA size range (100 bp-166 kbp) within 13 s, which are much wider and faster conditions than those of any existing methods. The network density is readily varied for the targeted DNA size range by tailoring the number of cycles of the nanowire growth only at the desired spatial position within the microchannel. The rigid dense 3D network structure with spatial density control plays an important role in determining the capability for analyzing DNA. Since the present method allows the spatial location and density of the nanostructure within the microchannels to be defined, this unique controllability offers a new strategy to develop an analytical method not only for DNA but also for other biological molecules.

Original languageEnglish
Article number5252
JournalScientific reports
Volume4
DOIs
Publication statusPublished - Jun 11 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

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