Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane

Toshio Hisatomi, Shoji Notomi, Takashi Tachibana, Yukio Sassa, Yasuhiro Ikeda, Takao Nakamura, Akifumi Ueno, Hiroshi Enaida, Toshinori Murata, Taiji Sakamoto, Tatsuro Ishibashi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. Design Laboratory investigation. Methods Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. Results The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed Müller cell processes were partially damaged, while regenerative spindle-shaped Müller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. Conclusions Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.

Original languageEnglish
JournalAmerican journal of ophthalmology
Volume158
Issue number3
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Membranes
Nerve Fibers
Neuroglia
Vitrectomy
Basement Membrane
Wound Healing
Retina
Scanning Transmission Electron Microscopy
Temazepam
Macaca fascicularis
Optic Nerve
Primates
Regeneration
Microscopy
Light

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane. / Hisatomi, Toshio; Notomi, Shoji; Tachibana, Takashi; Sassa, Yukio; Ikeda, Yasuhiro; Nakamura, Takao; Ueno, Akifumi; Enaida, Hiroshi; Murata, Toshinori; Sakamoto, Taiji; Ishibashi, Tatsuro.

In: American journal of ophthalmology, Vol. 158, No. 3, 01.01.2014.

Research output: Contribution to journalArticle

Hisatomi, Toshio ; Notomi, Shoji ; Tachibana, Takashi ; Sassa, Yukio ; Ikeda, Yasuhiro ; Nakamura, Takao ; Ueno, Akifumi ; Enaida, Hiroshi ; Murata, Toshinori ; Sakamoto, Taiji ; Ishibashi, Tatsuro. / Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane. In: American journal of ophthalmology. 2014 ; Vol. 158, No. 3.
@article{9ffe9b1b9f674cd0b2a1bdc169d77f7d,
title = "Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane",
abstract = "Purpose To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. Design Laboratory investigation. Methods Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. Results The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed M{\"u}ller cell processes were partially damaged, while regenerative spindle-shaped M{\"u}ller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. Conclusions Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.",
author = "Toshio Hisatomi and Shoji Notomi and Takashi Tachibana and Yukio Sassa and Yasuhiro Ikeda and Takao Nakamura and Akifumi Ueno and Hiroshi Enaida and Toshinori Murata and Taiji Sakamoto and Tatsuro Ishibashi",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.ajo.2014.05.022",
language = "English",
volume = "158",
journal = "American Journal of Ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
number = "3",

}

TY - JOUR

T1 - Ultrastructural changes of the vitreoretinal interface during long-term follow-up after removal of the internal limiting membrane

AU - Hisatomi, Toshio

AU - Notomi, Shoji

AU - Tachibana, Takashi

AU - Sassa, Yukio

AU - Ikeda, Yasuhiro

AU - Nakamura, Takao

AU - Ueno, Akifumi

AU - Enaida, Hiroshi

AU - Murata, Toshinori

AU - Sakamoto, Taiji

AU - Ishibashi, Tatsuro

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Purpose To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. Design Laboratory investigation. Methods Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. Results The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed Müller cell processes were partially damaged, while regenerative spindle-shaped Müller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. Conclusions Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.

AB - Purpose To investigate long-term ultrastructural changes in the retina after internal limiting membrane (ILM) peeling through the examination of morphologic changes 3 years after vitrectomy in cynomolgus monkeys. Design Laboratory investigation. Methods Pars plana vitrectomy was performed, followed by ILM peeling, in 2 primate eyes. Ultrastructural changes were investigated using light microscopy and transmission and scanning electron microscopy 3 years after ILM peeling. Results The remaining posterior vitreous and ILM-peeled areas were clearly recognized after the long-term follow-up. The exposed Müller cell processes were partially damaged, while regenerative spindle-shaped Müller cell processes developed, covering most of the retina. Notably, the nerve fiber layer was found to be uncovered and exposed to the vitreous space owing to misdirection of glial wound healing in some parts. In these areas, glial wound healing occurred beneath the nerve fiber layer. Although the glial cells covered the damaged areas, there was no apparent ILM regeneration in the shape of a continuous flat sheet, with the exception of accumulated deposits of basement membrane materials. Conclusions Although the retinal structures were well preserved after ILM peeling, ILM peeling resulted in mild damage to the vitreoretinal interface, which was not completely restored even after 3 years. The multilinear shape of the exposed nerve fiber may explain the previously reported dissociated optic nerve fiber layer appearance. The glial cells produced basement membrane materials around their processes, although they did not restore the ILM as a flat sheet.

UR - http://www.scopus.com/inward/record.url?scp=84908365946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908365946&partnerID=8YFLogxK

U2 - 10.1016/j.ajo.2014.05.022

DO - 10.1016/j.ajo.2014.05.022

M3 - Article

VL - 158

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

SN - 0002-9394

IS - 3

ER -