Ultrastructural localization of laminin-5 (γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy

Ikiru Atsuta, Takayoshi Yamaza, Masao Yoshinari, Tetsuya Goto, Mizuho A. Kido, Tadayoshi Kagiya, Satoya Mino, Masaki Shimono, Teruo Tanaka

Research output: Contribution to journalArticle

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Abstract

Laminin-5 (Ln-5) is an important molecule associated with epithelial cell adhesion and migration. In the gingiva around the tooth, Ln-5 localizes within basement membranes between the junctional epithelium (JE) and the tooth or connective tissue. Recently, we reported that in the oral mucosa around a dental implant, Ln-5 is expressed within the basement membranes at the implant-peri-implant epithelium (PIE) interface, and at the PIE-connective tissue interface. However, the ultrastructural localization of Ln-5 within or along the PIE has not yet been reported. Therefore, peri-implant oral mucosa was treated with anti-Ln-5 (γ2 chain) antibody and examined using immuno-electron microscopy. Ln-5 was localized in the cells of the innermost-third layer and basal layer of the PIE. A 100-nm-wide Ln-5-positive internal basal lamina (basement membrane) and hemidesmosomes as adhesion structures were formed at the apical portion of the implant-PIE interface. However, at the upper-middle portion of the interface, these adhesion structures were not observed. Furthermore, at the PIE-connective tissue interface, the Ln-5-positive external basal lamina (basement membrane) and hemidesmosomes were partially deficient. Judging from these findings, we concluded that Ln-5 contributes to the attachment of the PIE to the titanium surface, and that PIE attached to titanium at the apical portion of the dental implant-PIE interface.

Original languageEnglish
Pages (from-to)6280-6287
Number of pages8
JournalBiomaterials
Volume26
Issue number32
DOIs
Publication statusPublished - Nov 1 2005

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Dental prostheses
Dental Implants
Immunoelectron Microscopy
Mouth Mucosa
Titanium
Electron microscopy
Rats
Epithelium
Basement Membrane
Tissue
Hemidesmosomes
Connective Tissue
Adhesion
Cell adhesion
Tooth
Epithelial Attachment
Antibodies
kalinin
Mucous Membrane
Gingiva

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Ultrastructural localization of laminin-5 (γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy. / Atsuta, Ikiru; Yamaza, Takayoshi; Yoshinari, Masao; Goto, Tetsuya; Kido, Mizuho A.; Kagiya, Tadayoshi; Mino, Satoya; Shimono, Masaki; Tanaka, Teruo.

In: Biomaterials, Vol. 26, No. 32, 01.11.2005, p. 6280-6287.

Research output: Contribution to journalArticle

Atsuta, Ikiru ; Yamaza, Takayoshi ; Yoshinari, Masao ; Goto, Tetsuya ; Kido, Mizuho A. ; Kagiya, Tadayoshi ; Mino, Satoya ; Shimono, Masaki ; Tanaka, Teruo. / Ultrastructural localization of laminin-5 (γ2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy. In: Biomaterials. 2005 ; Vol. 26, No. 32. pp. 6280-6287.
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