Ultrastructure of the vitreoretinal interface following the removal of the internal limiting membrane using indocyanine green

Takao Nakamura, Toshinori Murata, Toshio Hisatomi, Hiroshi Enaida, Yukio Sassa, Akifumi Ueno, Taiji Sakamoto, Tatsuro Ishibashi

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Purpose. To investigate the morphological changes of the vitreoretinal interface following the removal of the internal limiting membrane (ILM) using indocyanine green (ICG). Methods. In 10 primate eyes, a pars plana vitrectomy was performed followed by ICG-assisted peeling of the ILM. Morphological changes in the exposed inner surface of the retina were investigated by electronmicroscopy immediately, 3, 6 and 12 months after the ILM removal. Results. The excised ILM was associated with fragments of glial tissue. The Muller cell processes were damaged and removed at the corresponding region of the retina. Regenerative spindle-shaped Muller cell processes were observed focally showing a meshwork like configuration at 3 and 6 months. Flattened and stretched processes were observed at 12 months; however, there was no apparent ILM regeneration. Conclusions. ICG assisted ILM peeling was observed to cause mild damage to the vitreoretinal interface, which did not completely recover within 12 months.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalCurrent Eye Research
Volume27
Issue number6
DOIs
Publication statusPublished - Dec 1 2003

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Indocyanine Green
Membranes
Ependymoglial Cells
Retina
Temazepam
Vitrectomy
Neuroglia
Primates
Regeneration

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ultrastructure of the vitreoretinal interface following the removal of the internal limiting membrane using indocyanine green. / Nakamura, Takao; Murata, Toshinori; Hisatomi, Toshio; Enaida, Hiroshi; Sassa, Yukio; Ueno, Akifumi; Sakamoto, Taiji; Ishibashi, Tatsuro.

In: Current Eye Research, Vol. 27, No. 6, 01.12.2003, p. 395-399.

Research output: Contribution to journalArticle

Nakamura, Takao ; Murata, Toshinori ; Hisatomi, Toshio ; Enaida, Hiroshi ; Sassa, Yukio ; Ueno, Akifumi ; Sakamoto, Taiji ; Ishibashi, Tatsuro. / Ultrastructure of the vitreoretinal interface following the removal of the internal limiting membrane using indocyanine green. In: Current Eye Research. 2003 ; Vol. 27, No. 6. pp. 395-399.
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AU - Enaida, Hiroshi

AU - Sassa, Yukio

AU - Ueno, Akifumi

AU - Sakamoto, Taiji

AU - Ishibashi, Tatsuro

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