Ultrathin films of charged polysaccharides assembled alternately with linear polyions

Yuri Lvov, Mitsuhiko Onda, Katsuhiko Ariga, Toyoki Kunitake

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

As a means of preparation of biocompatible molecular surfaces, an alternate assembly of charged polysaccharides and oppositely-charged synthetic polymers was conducted. Cationic chitosan was assembled alternately with anionic poly(sodium styrenesulfonate) (PSS) at pH 4. Regular film growth and its dependence on ionic strength were detected by the quartz crystal microbalance (QCM) method. Averaged film thicknesses for the chitosanCPSS layer were 15, 31, 46, and 69 A°, respectively, when 0, 0.25, 0.5, and 1 M of NaCl was contained in aqueous chitosan. Adsorption of chitosan did not reach saturation in 20 min at 0 M NaCl, while the adsorption became saturated within 6 min with 0.25 M NaCl. Anionic sodium chondroitin sulfate was also assembled in alternation with cationic poly(dimethyldiallylammonium chloride) (PDDA) at pH 6.5. The adsorption of chondroitin sulfate was less sensitive to ionic strength. Surface morphology of chitosan–PSS films was investigated by non-contact atomic force microscopy (AFM) observation. Maximum height difference and Ra value for a 1000 × 1000 nm area were 11 and 0.69 nm, respectively, indicating the formation of a molecularly flat surface by alternate layer-by-layer adsorption.

Original languageEnglish
Pages (from-to)345-355
Number of pages11
JournalJournal of Biomaterials Science, Polymer Edition
Volume9
Issue number4
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Ultrathin films
Polysaccharides
Adsorption
Chitosan
Chondroitin Sulfates
Ionic strength
Osmolar Concentration
Quartz Crystal Microbalance Techniques
Sodium sulfate
Atomic Force Microscopy
Quartz crystal microbalances
Film growth
Surface morphology
Film thickness
Chlorides
Atomic force microscopy
Polymers
Sodium
Observation
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Ultrathin films of charged polysaccharides assembled alternately with linear polyions. / Lvov, Yuri; Onda, Mitsuhiko; Ariga, Katsuhiko; Kunitake, Toyoki.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 9, No. 4, 01.01.1998, p. 345-355.

Research output: Contribution to journalArticle

Lvov, Yuri ; Onda, Mitsuhiko ; Ariga, Katsuhiko ; Kunitake, Toyoki. / Ultrathin films of charged polysaccharides assembled alternately with linear polyions. In: Journal of Biomaterials Science, Polymer Edition. 1998 ; Vol. 9, No. 4. pp. 345-355.
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