Ultraviolet excitation photothermal spectroscopy of non-labeled amino acids and visible light-induced signal enhancement

Satoshi Hirashima, Akira Harata

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    A measurement system for ultraviolet-laser excitation photothermal lens spectroscopy has been designed and constructed for measuring dilute amino acids in liquid solutions. An ultraviolet laser beam is generated as the fourth harmonic of a Ti:sapphire laser in the wavelength range of 212 to 220nm. Photothermal lens spectra of alanine, phenylalanine, serine, and tryptophan are observed. Photothermal lens spectra of these samples are coincident with their absorption spectra. It is demonstrated that photothermal lens signals of the amino acids can be amplified by simultaneous excitation with a visible laser. This ultraviolet-

    Original languageEnglish
    Pages (from-to)3970-3973
    Number of pages4
    JournalJapanese journal of applied physics
    Volume47
    Issue number5 PART 2
    DOIs
    Publication statusPublished - May 23 2008

    All Science Journal Classification (ASJC) codes

    • Engineering(all)
    • Physics and Astronomy(all)

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