Ultraviolet-laser excitation microscopic photothermal lens imaging for observing biological cells

Akira Harata, Takashi Matuda, Satoshi Hirashima

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    An ultraviolet-laser excitation microscopic photothermal lens imaging system for observing chemical compounds in biological cells has been designed and constructed, in which a reflection objective lens is used for focusing an excitation laser beam at 261 nm and a probe laser beam at 784 nm. Photothermal amplitude and phase images of yeast fungus cells are successfully obtained; together with a transmitted light image at 784 nm. The amplitude image represents photo-absorption at 261 nm by the cells The phase image seems to represent local thermal properties around the cells.

    Original languageEnglish
    Pages (from-to)4561-4563
    Number of pages3
    JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
    Volume46
    Issue number7 B
    DOIs
    Publication statusPublished - Jul 26 2007

    Fingerprint

    Ultraviolet lasers
    Laser excitation
    ultraviolet lasers
    Laser beams
    Lenses
    lenses
    Imaging techniques
    Chemical compounds
    Fungi
    Imaging systems
    Yeast
    excitation
    Thermodynamic properties
    cells
    laser beams
    chemical compounds
    fungi
    yeast
    photoabsorption
    thermodynamic properties

    All Science Journal Classification (ASJC) codes

    • Physics and Astronomy (miscellaneous)

    Cite this

    Ultraviolet-laser excitation microscopic photothermal lens imaging for observing biological cells. / Harata, Akira; Matuda, Takashi; Hirashima, Satoshi.

    In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 46, No. 7 B, 26.07.2007, p. 4561-4563.

    Research output: Contribution to journalArticle

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