Ultraviolet-laser excitation microscopic photothermal lens imaging for observing biological cells

Akira Harata, Takashi Matuda, Satoshi Hirashima

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    An ultraviolet-laser excitation microscopic photothermal lens imaging system for observing chemical compounds in biological cells has been designed and constructed, in which a reflection objective lens is used for focusing an excitation laser beam at 261 nm and a probe laser beam at 784 nm. Photothermal amplitude and phase images of yeast fungus cells are successfully obtained; together with a transmitted light image at 784 nm. The amplitude image represents photo-absorption at 261 nm by the cells The phase image seems to represent local thermal properties around the cells.

    Original languageEnglish
    Pages (from-to)4561-4563
    Number of pages3
    JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
    Volume46
    Issue number7 B
    DOIs
    Publication statusPublished - Jul 26 2007

    All Science Journal Classification (ASJC) codes

    • Engineering(all)
    • Physics and Astronomy(all)

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