Unexpected genomic features of high current density-producing Geobacter sulfurreducens strain YM18

Takashi Fujikawa, Yoshitoshi Ogura, Koki Ishigami, Yoshihiro Kawano, Miyuki Nagamine, Tetsuya Hayashi, Kengo Inoue

Research output: Contribution to journalArticlepeer-review

Abstract

Geobacter sulfurreducens produces high current densities and it has been used as a model organism for extracellular electron transfer studies. Nine G. sulfurreducens strains were isolated from biofilms formed on an anode poised at -0.2 V (vs SHE) in a bioelectrochemical system in which river sediment was used as an inoculum. The maximum current density of an isolate, strain YM18 (9.29 A/m2), was higher than that of the strain PCA (5.72 A/m2), the type strain of G. sulfurreducens, and comparable to strain KN400 (8.38 A/m2), which is another high current-producing strain of G. sulfurreducens. Genomic comparison of strains PCA, KN400 and YM18 revealed that omcB, xapD, spc and ompJ, which are known to be important genes for iron reduction and current production in PCA, were not present in YM18. In the PCA and KN400 genomes, two and one region(s) encoding CRISPR/Cas systems were identified, respectively, but they were missing in the YM18 genome. These results indicate that there is genetic variation in the key components involved in extracellular electron transfer among G. sulfurreducens strains.

Original languageEnglish
Article numberfnab119
JournalFEMS microbiology letters
Volume368
Issue number17
DOIs
Publication statusPublished - Sep 1 2021

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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