Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyoshi Matsumoto, Yutaka Osajima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.

Original languageEnglish
Pages (from-to)1295-1297
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number6
DOIs
Publication statusPublished - Jan 1 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide'. Together they form a unique fingerprint.

  • Cite this

    Ishikawa, H., Shimoda, M., Yonekura, A., Mishima, K., Matsumoto, K., & Osajima, Y. (2000). Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide. Bioscience, Biotechnology and Biochemistry, 64(6), 1295-1297. https://doi.org/10.1271/bbb.64.1295