Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase

Yasuhiro Horibata, Keishi Sakaguchi, Nozomu Okino, Hiroshi Iida, Masanori Inagaki, Toshitaka Fujisawa, Yoichiro Hama, Makoto Ito

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Endoglycoceramidase (EGCase; EC 3.2.1.123) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We detected strong EGCase activity in animals belonging to Cnidaria, Mollusca, and Annelida and cloned the enzyme from a hydra, Hydra magnipapillata. The hydra EGCase, consisting of 517 amino acid residues, showed 19.2% and 50.2% identity to the Rhodococcus and jellyfish EGCases, respectively. The recombinant hydra enzyme, expressed in CHOP (Chinese hamster ovary cells expressing polyoma LT antigen) cells, hydrolyzed [14C]GM1a to produce [14C]ceramide with a pH optimum at 3.0-3.5. Whole mount in situ hybridization and immunocytochemical analysis revealed that EGCase was widely expressed in the endodermal layer, especially in digestive cells. GM1a injected into the gastric cavity was incorporated and then directly catabolized by EGCase to produce GM1a-oligosaccharide and ceramide, which were further degraded by exoglycosidases and ceramidase, respectively. However, hydra exoglycosidases did not hydrolyze GM1a directly. These results indicate that the EGCase is indispensable for the catabolic processing of dietary glycosphingolipids in hydra, demonstrating the unique catabolic pathway for glycosphingolipids in the animal.

Original languageEnglish
Pages (from-to)33379-33389
Number of pages11
JournalJournal of Biological Chemistry
Volume279
Issue number32
DOIs
Publication statusPublished - Aug 6 2004

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endoglycoceramidase
Hydra
Glycosphingolipids
Ceramides
Glycoside Hydrolases
Oligosaccharides
Ceramidases
Animals
Enzymes
Annelida
Cells
Cnidaria
Rhodococcus
Antigens
Amino Acids
Mollusca
Processing
Cricetulus
In Situ Hybridization
Ovary

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase. / Horibata, Yasuhiro; Sakaguchi, Keishi; Okino, Nozomu; Iida, Hiroshi; Inagaki, Masanori; Fujisawa, Toshitaka; Hama, Yoichiro; Ito, Makoto.

In: Journal of Biological Chemistry, Vol. 279, No. 32, 06.08.2004, p. 33379-33389.

Research output: Contribution to journalArticle

Horibata, Yasuhiro ; Sakaguchi, Keishi ; Okino, Nozomu ; Iida, Hiroshi ; Inagaki, Masanori ; Fujisawa, Toshitaka ; Hama, Yoichiro ; Ito, Makoto. / Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 32. pp. 33379-33389.
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