Unusual feature of the T-CELL receptor genes in T-lineage acute lymphoblastic leukemia

Katsuo Yamanaka, Kazuaki Kubo, Hitoshi Kiyoi, Takuji Ichihashi, Koji Kato, Keizo Horibe, Tomoki Naoe

Research output: Contribution to journalArticle

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Abstract

We characterized the T-cell receptor (TCR) gene rearrangements and sequences in 15 T-lineage acute lymphoblastic leukemia (T-ALL) and seven adult T-cell leukemia (ATL) samples. Southern blot analysis showed that neither of the two TCR δ alleles was deleted in two T-ALL samples, suggesting that the TCR α loci have a germ line configuration. The TCR α and β sequences were cloned and sequenced by reverse transcriptase-inverse polymerase chain reaction. Two T-ALL samples had a long complementarity determining region (CDR), three of the α chain and the other two T-ALL samples had long CDR3 of the β chain, compared with normal peripheral blood lymphocytes (PBL). Thus, a total of six T-ALL samples had unusual TCR gene structure, which was unrelated to the immunophenotype. On the other hand, CDR3 length in ATL samples was similar to normal PBL. These data suggest that T-ALL is derived from an immature T-cell repertoire which undergoes TCR gene rearrangement or has not been negatively selected.

Original languageEnglish
Pages (from-to)667-674
Number of pages8
JournalLeukemia Research
Volume21
Issue number7
DOIs
Publication statusPublished - Jul 1 1997
Externally publishedYes

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
T-Cell Receptor Genes
T-Cell Antigen Receptor
T-Lymphocyte Gene Rearrangement
Genes
Adult T Cell Leukemia Lymphoma
Lymphocytes
Complementarity Determining Regions
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Germ Cells
Alleles
T-Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Unusual feature of the T-CELL receptor genes in T-lineage acute lymphoblastic leukemia. / Yamanaka, Katsuo; Kubo, Kazuaki; Kiyoi, Hitoshi; Ichihashi, Takuji; Kato, Koji; Horibe, Keizo; Naoe, Tomoki.

In: Leukemia Research, Vol. 21, No. 7, 01.07.1997, p. 667-674.

Research output: Contribution to journalArticle

Yamanaka, K, Kubo, K, Kiyoi, H, Ichihashi, T, Kato, K, Horibe, K & Naoe, T 1997, 'Unusual feature of the T-CELL receptor genes in T-lineage acute lymphoblastic leukemia', Leukemia Research, vol. 21, no. 7, pp. 667-674. https://doi.org/10.1016/S0145-2126(97)00039-8
Yamanaka, Katsuo ; Kubo, Kazuaki ; Kiyoi, Hitoshi ; Ichihashi, Takuji ; Kato, Koji ; Horibe, Keizo ; Naoe, Tomoki. / Unusual feature of the T-CELL receptor genes in T-lineage acute lymphoblastic leukemia. In: Leukemia Research. 1997 ; Vol. 21, No. 7. pp. 667-674.
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AU - Kato, Koji

AU - Horibe, Keizo

AU - Naoe, Tomoki

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AB - We characterized the T-cell receptor (TCR) gene rearrangements and sequences in 15 T-lineage acute lymphoblastic leukemia (T-ALL) and seven adult T-cell leukemia (ATL) samples. Southern blot analysis showed that neither of the two TCR δ alleles was deleted in two T-ALL samples, suggesting that the TCR α loci have a germ line configuration. The TCR α and β sequences were cloned and sequenced by reverse transcriptase-inverse polymerase chain reaction. Two T-ALL samples had a long complementarity determining region (CDR), three of the α chain and the other two T-ALL samples had long CDR3 of the β chain, compared with normal peripheral blood lymphocytes (PBL). Thus, a total of six T-ALL samples had unusual TCR gene structure, which was unrelated to the immunophenotype. On the other hand, CDR3 length in ATL samples was similar to normal PBL. These data suggest that T-ALL is derived from an immature T-cell repertoire which undergoes TCR gene rearrangement or has not been negatively selected.

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