TY - JOUR
T1 - Up-regulation of CD44 expression by tumor necrosis factor-α is neutralized by interleukin-10 in Langerhans cells
AU - Osada, A.
AU - Nakashima, H.
AU - Furue, M.
AU - Tamaki, K.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - CD44 is a principal cell-surface receptor for hyaluronate and is found on a wide variety of cells. CD44 plays an important role in lymphocyte homing, lymphohemopoiesis, and T-cell activation as well as in cell motility and migration, CD44 is expressed on the cell surface of epidermal langerhans cells (LC), and is one of the candidates for molecules that are involved in the migratory capability of LC, but little is known about its regulatory properties. We examined the modulatory effects of tumor necrosis factor (TNF)-α and interleukin (IL)-10 on the CD44 expression in LC. We found 1) that TNF-α significantly up-regulated the expression of CD44 in a concentration-dependent manner, 2) that IL-10 down-regulated the expression of CD44 in a concentration-dependent manner, 3) that the effect of TNF-α or IL-10 was readily detectable as early as 24 h after the initiation of culture, and 4) that the simultaneous addition of TNF-α and IL-10 mutually neutralized the effect of each other. These data suggest that in the epidermal microenvironment the expression of CD44 in LC may be reciprocally regulated by TNF-α and IL-10, both of which are known to be produced by surrounding keratinocytes.
AB - CD44 is a principal cell-surface receptor for hyaluronate and is found on a wide variety of cells. CD44 plays an important role in lymphocyte homing, lymphohemopoiesis, and T-cell activation as well as in cell motility and migration, CD44 is expressed on the cell surface of epidermal langerhans cells (LC), and is one of the candidates for molecules that are involved in the migratory capability of LC, but little is known about its regulatory properties. We examined the modulatory effects of tumor necrosis factor (TNF)-α and interleukin (IL)-10 on the CD44 expression in LC. We found 1) that TNF-α significantly up-regulated the expression of CD44 in a concentration-dependent manner, 2) that IL-10 down-regulated the expression of CD44 in a concentration-dependent manner, 3) that the effect of TNF-α or IL-10 was readily detectable as early as 24 h after the initiation of culture, and 4) that the simultaneous addition of TNF-α and IL-10 mutually neutralized the effect of each other. These data suggest that in the epidermal microenvironment the expression of CD44 in LC may be reciprocally regulated by TNF-α and IL-10, both of which are known to be produced by surrounding keratinocytes.
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U2 - 10.1111/1523-1747.ep12313437
DO - 10.1111/1523-1747.ep12313437
M3 - Article
C2 - 7542295
AN - SCOPUS:0029092675
VL - 105
SP - 124
EP - 127
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 1
ER -