Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina

Kazuhiro Tokuda, Yasuhiro Kuramitsu, Baron Byron, Takao Kitagawa, Nobuko Tokuda, Daiki Kobayashi, Megumi Nagayama, Norie Araki, Kohei Sonoda, Kazuyuki Nakamura

Research output: Contribution to journalArticle

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Abstract

Glutamate has been shown to induce neural progenitor cells in the adult vertebrate retina. However, protein dynamics during progenitor cell induction by glutamate are not fully understood. To identify specific proteins involved in the process, we employed two-dimensional electrophoresis-based proteomics on glutamate untreated and treated retinal ex vivo sections. Rat retinal tissues were incubated with 1 mM glutamate for 1 h, followed by incubation in glutamate-free media for a total of 24 h. Consistent with prior reports, it was found that mitotic cells appeared in the outer nuclear layer without any histological damage. Immunohistological evaluations and immunoblotting confirmed the emergence of neuronal progenitor cells in the mature retina treated with glutamate. Proteomic analysis revealed the up-regulation of dihydropyrimidinase-related protein 3 (DRP-3), DRP-2 and stress-induced-phosphoprotein 1 (STIP1) during neural progenitor cell induction by glutamate. Moreover, mRNA expression of DRP-3, especially, its long isoform, robustly increased in the treated retina compared to that in the untreated retina. These results may indicate that glutamate induces neural progenitor cells in the mature rat retina by up-regulating the proteins which mediate cell mitosis and neurite growth.

Original languageEnglish
Pages (from-to)593-599
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume463
Issue number4
DOIs
Publication statusPublished - Jul 13 2015
Externally publishedYes

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dihydropyrimidinase
Retina
Rats
Glutamic Acid
Protein Isoforms
Up-Regulation
Stem Cells
Proteins
Proteomics
Phosphoproteins
Neurites
Electrophoresis
Immunoblotting
Mitosis
Vertebrates

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. / Tokuda, Kazuhiro; Kuramitsu, Yasuhiro; Byron, Baron; Kitagawa, Takao; Tokuda, Nobuko; Kobayashi, Daiki; Nagayama, Megumi; Araki, Norie; Sonoda, Kohei; Nakamura, Kazuyuki.

In: Biochemical and Biophysical Research Communications, Vol. 463, No. 4, 13.07.2015, p. 593-599.

Research output: Contribution to journalArticle

Tokuda, Kazuhiro ; Kuramitsu, Yasuhiro ; Byron, Baron ; Kitagawa, Takao ; Tokuda, Nobuko ; Kobayashi, Daiki ; Nagayama, Megumi ; Araki, Norie ; Sonoda, Kohei ; Nakamura, Kazuyuki. / Up-regulation of DRP-3 long isoform during the induction of neural progenitor cells by glutamate treatment in the ex vivo rat retina. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 463, No. 4. pp. 593-599.
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