Upregulation of cytochrome p450s following exposure of the silkmoth, Bombyx mori to insecticides

Research output: Contribution to journalArticle

Abstract

We examined which proteins were enhanced in the silkmoth in response to its exposure to insecticides. We previously found that the levels of four cytochrome P450s (CYPs) were higher following insecticide exposure (Yamamoto et al., 2010). In this study, we focused on other mRNAs encoding the silkmoth CYP proteins (CYP6B29, CYP18A1, CYP366A1, CYP302A1) induced. mRNA expression analysis revealed that each CYP mRNA was expressed in a tissue-specifc manner and that expression was developmentally regulated. Quantitative PCR revealed that these CYP mRNAs were upregulated in the larval fat body after exposure of the silkmoth to various insecticides. Our results suggest that the CYPs can contribute to insecticide resistance in Lepidopterans.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalJournal of Insect Biotechnology and Sericology
Volume82
Issue number2
DOIs
Publication statusPublished - Dec 12 2013

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Insecticides
Bombyx
Cytochromes
Bombyx mori
cytochromes
Up-Regulation
insecticides
Proteins
Messenger RNA
Insecticide Resistance
Fat Body
insecticide resistance
fat body
quantitative polymerase chain reaction
proteins
Lepidoptera
Oils and fats
Polymerase Chain Reaction
Tissue
Protein

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

Cite this

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title = "Upregulation of cytochrome p450s following exposure of the silkmoth, Bombyx mori to insecticides",
abstract = "We examined which proteins were enhanced in the silkmoth in response to its exposure to insecticides. We previously found that the levels of four cytochrome P450s (CYPs) were higher following insecticide exposure (Yamamoto et al., 2010). In this study, we focused on other mRNAs encoding the silkmoth CYP proteins (CYP6B29, CYP18A1, CYP366A1, CYP302A1) induced. mRNA expression analysis revealed that each CYP mRNA was expressed in a tissue-specifc manner and that expression was developmentally regulated. Quantitative PCR revealed that these CYP mRNAs were upregulated in the larval fat body after exposure of the silkmoth to various insecticides. Our results suggest that the CYPs can contribute to insecticide resistance in Lepidopterans.",
author = "Kohji Yamamoto and Hirofumi Ichinose and Yoichi Aso and Miyako Udono and Yoshinori Katakura",
year = "2013",
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language = "English",
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pages = "33--38",
journal = "Journal of Insect Biotechnology and Sericology",
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T1 - Upregulation of cytochrome p450s following exposure of the silkmoth, Bombyx mori to insecticides

AU - Yamamoto, Kohji

AU - Ichinose, Hirofumi

AU - Aso, Yoichi

AU - Udono, Miyako

AU - Katakura, Yoshinori

PY - 2013/12/12

Y1 - 2013/12/12

N2 - We examined which proteins were enhanced in the silkmoth in response to its exposure to insecticides. We previously found that the levels of four cytochrome P450s (CYPs) were higher following insecticide exposure (Yamamoto et al., 2010). In this study, we focused on other mRNAs encoding the silkmoth CYP proteins (CYP6B29, CYP18A1, CYP366A1, CYP302A1) induced. mRNA expression analysis revealed that each CYP mRNA was expressed in a tissue-specifc manner and that expression was developmentally regulated. Quantitative PCR revealed that these CYP mRNAs were upregulated in the larval fat body after exposure of the silkmoth to various insecticides. Our results suggest that the CYPs can contribute to insecticide resistance in Lepidopterans.

AB - We examined which proteins were enhanced in the silkmoth in response to its exposure to insecticides. We previously found that the levels of four cytochrome P450s (CYPs) were higher following insecticide exposure (Yamamoto et al., 2010). In this study, we focused on other mRNAs encoding the silkmoth CYP proteins (CYP6B29, CYP18A1, CYP366A1, CYP302A1) induced. mRNA expression analysis revealed that each CYP mRNA was expressed in a tissue-specifc manner and that expression was developmentally regulated. Quantitative PCR revealed that these CYP mRNAs were upregulated in the larval fat body after exposure of the silkmoth to various insecticides. Our results suggest that the CYPs can contribute to insecticide resistance in Lepidopterans.

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