Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney

Kazushige Takahashi, Satohiro Masuda, Nobuhiko Nakamura, Hideyuki Saito, Takahiro Futami, Toshio Doi, Ken Ichi Inui

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The progression of renal damage resulting from reduced nephron mass has been extensively studied in the 5/6 nephrectomized rat. However, reabsorption of small peptides and D-glucose across the renal proximal tubule in this model remains poorly understood. In this study, we examined the alterations of H + -peptide cotransporters (PEPT1 and PEPT2) and Na + -D-glucose cotransporters (SGLT1 and SGLT2) in chronic renal failure. Two weeks after surgery, H + -dependent [ 14 C]glycylsarcosine uptake by the renal brush-border membrane vesicles isolated from 5/6 nephrectomized rats was significantly increased compared with that from sham-operated controls. Kinetic analysis revealed that the maximum velocity value for [ 14 C]glycylsarcosine uptake by the high-affinity-type of peptide transporter was increased threefold by 5/6 nephrectomy, without significant changes in the apparent Michaelis-Menten constant value. Competitive PCR analyses indicated that the expression of PEPT2 mRNA was markedly increased in the remnant kidney, but PEPT1, SGLT1, and SGLT2 mRNA levels showed no significant changes. These findings indicated that the high-affinity-type H + -peptide cotransport activity is upregulated by 5/6 nephrectomy, accompanied by the increased expression of PEPT2. The upregulation of PEPT2 expression would result in an increase in reabsorption of small peptides and peptide-like drugs across the brush-border membranes in chronic renal failure.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number6 50-6
Publication statusPublished - Dec 1 2001
Externally publishedYes

Fingerprint

Up-Regulation
Kidney
Peptides
Microvilli
Nephrectomy
Chronic Kidney Failure
Glucose
Proximal Kidney Tubule
Messenger RNA
Membranes
Nephrons
Polymerase Chain Reaction
hydrogen-coupled oligopeptide transporter PepT2
Pharmaceutical Preparations
glycylsarcosine

All Science Journal Classification (ASJC) codes

  • Physiology
  • Urology

Cite this

Takahashi, K., Masuda, S., Nakamura, N., Saito, H., Futami, T., Doi, T., & Inui, K. I. (2001). Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney American Journal of Physiology - Renal Physiology, 281(6 50-6).

Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney . / Takahashi, Kazushige; Masuda, Satohiro; Nakamura, Nobuhiko; Saito, Hideyuki; Futami, Takahiro; Doi, Toshio; Inui, Ken Ichi.

In: American Journal of Physiology - Renal Physiology, Vol. 281, No. 6 50-6, 01.12.2001.

Research output: Contribution to journalArticle

Takahashi, K, Masuda, S, Nakamura, N, Saito, H, Futami, T, Doi, T & Inui, KI 2001, ' Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney ', American Journal of Physiology - Renal Physiology, vol. 281, no. 6 50-6.
Takahashi K, Masuda S, Nakamura N, Saito H, Futami T, Doi T et al. Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney American Journal of Physiology - Renal Physiology. 2001 Dec 1;281(6 50-6).
Takahashi, Kazushige ; Masuda, Satohiro ; Nakamura, Nobuhiko ; Saito, Hideyuki ; Futami, Takahiro ; Doi, Toshio ; Inui, Ken Ichi. / Upregulation of H + -peptide cotransporter PEPT2 in rat remnant kidney In: American Journal of Physiology - Renal Physiology. 2001 ; Vol. 281, No. 6 50-6.
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