Upregulation of P-glycoprotein activity in porcine oocytes and granulosa cells during in vitro maturation

Kazuko Yokota, Takuro Hirano, Narumi Urata, Nobuhiko Yamauchi, Masa Aki Hattori

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Multidrug resistance P-glycoprotein (Pgp), coded by the multidrug resistance type I (MDR1/ABCB1) gene, is an energy-dependent efflux pump and functions in systemic detoxification processes. In the present study, the expression and development of Pgp were evaluated in the porcine oocyte during in vitro maturation to compare with the expression of Pgp in cultured granulosa cells. As revealed by Western blotting using anti-human Pgp antibody, a single band of Pgp with an apparent molecular size of 170 kDa was detected in the germinal vesicle stage oocytes. The surface of GV oocyte was positively labeled by immunostaining. In the second metaphase oocyte after culture in the maturation medium containing porcine follicular fluid and human chorionic gonadotropin, the level of Pgp was increased. The elevation of the oocyte Pgp level was associated with increased activity of rhodamine 6G efflux from the oocyte, and its efflux was suppressed by verapamil, an inhibitor of Pgp. Removal of porcine follicular fluid from the maturation medium resulted in little alteration of the oocyte Pgp level. Expression of Pgp was also elevated in cultured porcine granulosa cells during cell maturation when stimulated with follicle-stimulating hormone or luteinizing hormone for 24-48 h. Collectively, the present results indicate that the transporting activity of P-glycoprotein upregulates in porcine oocytes and granulosa cells during exposure to gonadotropins or prior to ovulation.

Original languageEnglish
Pages (from-to)322-326
Number of pages5
JournalJournal of Reproduction and Development
Volume57
Issue number3
DOIs
Publication statusPublished - Jun 1 2011

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granulosa cells
oocytes
swine
follicular fluid
multiple drug resistance
P-glycoproteins
germinal vesicle
verapamil
human chorionic gonadotropin
follicle-stimulating hormone
gonadotropins
luteinizing hormone
metaphase
cultured cells
transporters
ovulation
Western blotting

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Upregulation of P-glycoprotein activity in porcine oocytes and granulosa cells during in vitro maturation. / Yokota, Kazuko; Hirano, Takuro; Urata, Narumi; Yamauchi, Nobuhiko; Hattori, Masa Aki.

In: Journal of Reproduction and Development, Vol. 57, No. 3, 01.06.2011, p. 322-326.

Research output: Contribution to journalArticle

Yokota, Kazuko ; Hirano, Takuro ; Urata, Narumi ; Yamauchi, Nobuhiko ; Hattori, Masa Aki. / Upregulation of P-glycoprotein activity in porcine oocytes and granulosa cells during in vitro maturation. In: Journal of Reproduction and Development. 2011 ; Vol. 57, No. 3. pp. 322-326.
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