Use of DNA strand damage (Comet assay) and embryo hatching effects to assess contaminant exposure in blue crab (Callinectes sapidus) embryos

Richard F. Lee, Scott A. Steinert, K. Nakayama, Yugi Oshima

Research output: Contribution to journalConference article

10 Citations (Scopus)

Abstract

After fertilization, blue crab eggs are embedded in a `sponge' which is attached to the female abdomen during embryo development. Embryos after 9 stages in the egg sac hatch into a swimming zoea stage (stage 10). We have developed a bioassay where embryo development is monitored in culture plates with and without toxicants in the water. Toxicant effects are based on determining the percentage of embryos which hatch to zoea. Hatching EC50 (toxicant concentration at which 50% of the embryos fail to hatch) for a number of pesticides, organometallics and metals were determined. The test takes from 2 to 6 days depending on the embryo stage selected for the study. In addition to embryo development effects the prevalence of DNA single-strand breaks in individual embryo cells were determined using the single cell gel electrophoresis method (Comet assay). A good correlation between DNA strand breakage and embryo defects was found after exposure to genotoxic contaminants. Thus, the bioassay linking DNA damage to embryo hatching effects is rapid, sensitive and mechanistically relevant.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalASTM Special Technical Publication
Issue number1364
Publication statusPublished - Dec 1 1999
EventProceedings of the 1998 8th Symposium on Environmental Toxicology and Risk Assessment: Standardization of Biomarkers for Endocrine Disruption and Environmental Assessment - Atlanta, GA, USA
Duration: Apr 20 1998Apr 22 1998

Fingerprint

Hatches
comet
hatching
embryo
crab
Assays
DNA
Bioassay
assay
Impurities
damage
pollutant
Organometallics
Pesticides
Electrophoresis
Gels
Defects
bioassay
Metals
effect

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Use of DNA strand damage (Comet assay) and embryo hatching effects to assess contaminant exposure in blue crab (Callinectes sapidus) embryos. / Lee, Richard F.; Steinert, Scott A.; Nakayama, K.; Oshima, Yugi.

In: ASTM Special Technical Publication, No. 1364, 01.12.1999, p. 341-349.

Research output: Contribution to journalConference article

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