TY - JOUR
T1 - Use of Membrane Potential to Achieve Transmembrane Modification with an Artificial Receptor
AU - Hatanaka, Wataru
AU - Kawaguchi, Miki
AU - Sun, Xizheng
AU - Nagao, Yusuke
AU - Ohshima, Hiroyuki
AU - Hashida, Mitsuru
AU - Higuchi, Yuriko
AU - Kishimura, Akihiro
AU - Katayama, Yoshiki
AU - Mori, Takeshi
N1 - Funding Information:
This work was financially supported by a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology of Japan (grant no. 16H04167).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/2/15
Y1 - 2017/2/15
N2 - We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.
AB - We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.
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U2 - 10.1021/acs.bioconjchem.6b00449
DO - 10.1021/acs.bioconjchem.6b00449
M3 - Article
C2 - 28052667
AN - SCOPUS:85013080676
SN - 1043-1802
VL - 28
SP - 296
EP - 301
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 2
ER -