Use of Membrane Potential to Achieve Transmembrane Modification with an Artificial Receptor

Wataru Hatanaka, Miki Kawaguchi, Xizheng Sun, Yusuke Nagao, Hiroyuki Ohshima, Mitsuru Hashida, Yuriko Higuchi, Akihiro Kishimura, Yoshiki Katayama, Takeshi Mori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.

Original languageEnglish
Pages (from-to)296-301
Number of pages6
JournalBioconjugate Chemistry
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 15 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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