Use of on-line supercritical fluid extraction-supercritical fluid chromatography/tandem mass spectrometry to analyze disease biomarkers in dried serum spots compared with serum analysis using liquid chromatography/tandem mass spectrometry

Makoto Suzuki, Shin Nishiumi, Takashi Kobayashi, Arata Sakai, Yosuke Iwata, Takato Uchikata, Yoshihiro Izumi, Takeshi Azuma, Takeshi Bamba, Masaru Yoshida

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Rationale: The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS. Methods: The chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns. Then, we optimized the SFE-SFC/MS/MS method using the diol column, focusing on candidate biomarkers of oral, colorectal, and pancreatic cancer that were identified using liquid chromatography (LC)/MS/MS. Results: By using this system, four hydrophilic metabolites and 17 hydrophobic metabolites were simultaneously detected within 15 min. In an experiment involving clinical samples, PC 16:0-18:2/16:1-18:1 exhibited 93.8% sensitivity and 64.3% specificity, whereas PC 17:1-18:1/17:0-18:2 showed 81.3% sensitivity and 92.9% specificity for detecting oral cancer. In addition, assessments of the creatine levels demonstrated 92.3% sensitivity and 78.6% specificity for detecting colorectal cancer. Conclusions: The results of this study indicate that our method has great potential for clinical diagnosis and would be suitable for large-scale screening.

Original languageEnglish
Pages (from-to)886-894
Number of pages9
JournalRapid Communications in Mass Spectrometry
Volume31
Issue number10
DOIs
Publication statusPublished - May 30 2017

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Supercritical fluids
Liquid chromatography
Biomarkers
Mass spectrometry
Metabolites
Online systems
Creatine
Assays
Screening
Throughput
Supercritical Fluid Chromatography
Experiments

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Cite this

Use of on-line supercritical fluid extraction-supercritical fluid chromatography/tandem mass spectrometry to analyze disease biomarkers in dried serum spots compared with serum analysis using liquid chromatography/tandem mass spectrometry. / Suzuki, Makoto; Nishiumi, Shin; Kobayashi, Takashi; Sakai, Arata; Iwata, Yosuke; Uchikata, Takato; Izumi, Yoshihiro; Azuma, Takeshi; Bamba, Takeshi; Yoshida, Masaru.

In: Rapid Communications in Mass Spectrometry, Vol. 31, No. 10, 30.05.2017, p. 886-894.

Research output: Contribution to journalArticle

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AU - Kobayashi, Takashi

AU - Sakai, Arata

AU - Iwata, Yosuke

AU - Uchikata, Takato

AU - Izumi, Yoshihiro

AU - Azuma, Takeshi

AU - Bamba, Takeshi

AU - Yoshida, Masaru

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