TY - JOUR
T1 - Use of on-line supercritical fluid extraction-supercritical fluid chromatography/tandem mass spectrometry to analyze disease biomarkers in dried serum spots compared with serum analysis using liquid chromatography/tandem mass spectrometry
AU - Suzuki, Makoto
AU - Nishiumi, Shin
AU - Kobayashi, Takashi
AU - Sakai, Arata
AU - Iwata, Yosuke
AU - Uchikata, Takato
AU - Izumi, Yoshihiro
AU - Azuma, Takeshi
AU - Bamba, Takeshi
AU - Yoshida, Masaru
N1 - Funding Information:
This study was supported in part by a grant from the Development of Systems and Technology for Advanced Measurement and Analysis Project (JST) [M.S., Y.I., T.U., Y.I., T.B., and M.Y.]; a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [M.Y.]; a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [S.N.]; a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [T.K.]; and the AMED-CREST from the Japan Agency for Medical Research and Development (AMED) [S.N., T.K., T.A., and M.Y.].
Publisher Copyright:
Copyright © 2017 John Wiley & Sons, Ltd.
PY - 2017/5/30
Y1 - 2017/5/30
N2 - Rationale: The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS. Methods: The chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns. Then, we optimized the SFE-SFC/MS/MS method using the diol column, focusing on candidate biomarkers of oral, colorectal, and pancreatic cancer that were identified using liquid chromatography (LC)/MS/MS. Results: By using this system, four hydrophilic metabolites and 17 hydrophobic metabolites were simultaneously detected within 15 min. In an experiment involving clinical samples, PC 16:0-18:2/16:1-18:1 exhibited 93.8% sensitivity and 64.3% specificity, whereas PC 17:1-18:1/17:0-18:2 showed 81.3% sensitivity and 92.9% specificity for detecting oral cancer. In addition, assessments of the creatine levels demonstrated 92.3% sensitivity and 78.6% specificity for detecting colorectal cancer. Conclusions: The results of this study indicate that our method has great potential for clinical diagnosis and would be suitable for large-scale screening.
AB - Rationale: The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS. Methods: The chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns. Then, we optimized the SFE-SFC/MS/MS method using the diol column, focusing on candidate biomarkers of oral, colorectal, and pancreatic cancer that were identified using liquid chromatography (LC)/MS/MS. Results: By using this system, four hydrophilic metabolites and 17 hydrophobic metabolites were simultaneously detected within 15 min. In an experiment involving clinical samples, PC 16:0-18:2/16:1-18:1 exhibited 93.8% sensitivity and 64.3% specificity, whereas PC 17:1-18:1/17:0-18:2 showed 81.3% sensitivity and 92.9% specificity for detecting oral cancer. In addition, assessments of the creatine levels demonstrated 92.3% sensitivity and 78.6% specificity for detecting colorectal cancer. Conclusions: The results of this study indicate that our method has great potential for clinical diagnosis and would be suitable for large-scale screening.
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U2 - 10.1002/rcm.7857
DO - 10.1002/rcm.7857
M3 - Article
C2 - 28332299
AN - SCOPUS:85018516450
SN - 0951-4198
VL - 31
SP - 886
EP - 894
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 10
ER -
