Use of RAPD markers for cultivar identification in Rhus succedanea L. J.

S. Goto, Atsushi Watanabe, K. Ikeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

There are many cultivars of Rhus succedanea L. in Japan. It is very difficult to distinguish them precisely on the basis of morphological traits, as these traits are influenced by environmental factors. In order to distinguish these cultivars more accurately from each other, RAPD (random amplified polymorphic DNA) markers were used in this study. Out of 140 primers screened, 9 primers gave 14 markers for cultivar identification. Seven cultivars having 19 plants were investigated for their genotypic identity by using these markers. RAPD patterns were unique for each cultivar. On the other hand, RAPD patterns of all ramets from individual ortets were identical. It is apparent from these studies, that RAPD markers can be used to identify Rhus succedanea L. cultivars. The cluster analysis based on genetic distances were also carried out to investigate the genetic relationship among cultivars. It was found that the cultivars originating in the close proximity to each other are more closely related at DNA level than the rest.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume79
Issue number4
Publication statusPublished - Dec 1 1997

All Science Journal Classification (ASJC) codes

  • Forestry

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