Usefulness and clinical significance of quantitative real-time RT-PCR to detect isolated tumor cells in the peripheral blood and tumor drainage blood of patients with colorectal cancer

Hisae Iinuma, Kota Okinaga, Hiroshi Egami, Koshi Mimori, Naoko Hayashi, Koujiro Nishida, Miki Adachi, Masaki Mori, Mitsuru Sasako

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Abstract

The clinical significance of isolated tumor cells (ITC) circulating in the blood of patients with colorectal cancer is unclear. In this study, we investigated the relationship between the presence of ITC that express carcinoembryonic antigen (CEA) and/or cytokeratin 20 (CK20) transcripts in the blood and the clinicopathological findings and prognosis using the quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. We studied peripheral blood and tumor drainage blood from 167 patients with colorectal cancer. Quantitative real-time RT-PCR assay was able to detect one tumor cell in 3×106 peripheral blood mononuclear cells. Applying a cut-off value, CEA and/or CK20 (CEA/CK20) were detected in 10.2% (17/167) of the patients' preoperative peripheral blood samples and 34.1% (57/167) of the patients' tumor drainage blood samples. In the relationship between the CEA/CK20 of the blood and the clinicopathological factors, a significant correlation was demonstrated between the positivity of marker genes and the depth of invasion, venous invasion, lymph node metastasis, liver metastasis or stage. The disease-free and overall survival of patients with CEA/CK20-positive peripheral or tumor drainage blood was significantly shorter than that of marker gene-negative patients. CEA/CK20 transcripts in tumor drainage blood were independent factors for prognosis in disease-free survival and overall survival. These results suggest that detecting CEA/CK20 mRNA in tumor drainage blood by real-time RT-PCR has prognostic value in patients with colorectal cancer. Large scale and longterm clinical studies are needed to confirm the prognostic value of genetically detecting ITC in the peripheral blood.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalInternational Journal of Oncology
Volume28
Issue number2
Publication statusPublished - Feb 1 2006

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Reverse Transcription
Drainage
Colorectal Neoplasms
Keratin-20
Polymerase Chain Reaction
Carcinoembryonic Antigen
Neoplasms
Disease-Free Survival
Neoplasm Metastasis
Circulating Neoplastic Cells
Genes
Blood Cells
Lymph Nodes
Messenger RNA
Survival
Liver

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Usefulness and clinical significance of quantitative real-time RT-PCR to detect isolated tumor cells in the peripheral blood and tumor drainage blood of patients with colorectal cancer. / Iinuma, Hisae; Okinaga, Kota; Egami, Hiroshi; Mimori, Koshi; Hayashi, Naoko; Nishida, Koujiro; Adachi, Miki; Mori, Masaki; Sasako, Mitsuru.

In: International Journal of Oncology, Vol. 28, No. 2, 01.02.2006, p. 297-306.

Research output: Contribution to journalArticle

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