TY - JOUR
T1 - Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer
AU - Sato, Nobutaka
AU - Hayashi, Naoko
AU - Imamura, Yu
AU - Tanaka, Yohei
AU - Kinoshita, Koichi
AU - Kurashige, Jyunji
AU - Saito, Seiya
AU - Karashima, Ryuichi
AU - Hirashima, Kotaro
AU - Nagai, Yohei
AU - Miyamoto, Yuji
AU - Iwatsuki, Masaaki
AU - Baba, Yoshifumi
AU - Watanabe, Masayuki
AU - Baba, Hideo
PY - 2012/6
Y1 - 2012/6
N2 - Purpose. The CellSearch system (Veridex, LLC) is useful for detecting circulating tumor cells (CTCs) in various carcinomas, including colorectal cancer (CRC); however, there are some problems associated with its clinical use. A transcription-reverse transcription concerted reaction (TRC) method, which is a PCR-based technique producing more stable and reliable results, because it is a more simplified process compared with the conventional techniques, has been introduced for detecting micrometastasis in some carcinomas. We aimed to demonstrate the effectiveness of TRC method in the CTC detection. Methods. We compared the two methods for the sensitivity for CTC detection using the colon cancer cell line and 42 whole-blood samples from patients with advanced or metastatic CRC. Furthermore, 25 patients with metastatic CRC were enrolled to investigate the correlation between CTC detection and prognosis in both methods. Results. The sensitivity of the TRC method was similar to that of the CellSearch system. The overall survival rate was significantly worse in the patients diagnosed as CTCpositive by the TRC method than in those diagnosed as CTC-negative; this finding was similar to the prognosis indicated by the CellSearch system. However, clinically, the TRC method could detect CTCs more rapidly and at a reduced cost compared with the CellSearch system. Conclusions. The TRC method seems to be a useful alternative to the CellSearch system for clinically detecting CTCs in patients with metastatic CRC.
AB - Purpose. The CellSearch system (Veridex, LLC) is useful for detecting circulating tumor cells (CTCs) in various carcinomas, including colorectal cancer (CRC); however, there are some problems associated with its clinical use. A transcription-reverse transcription concerted reaction (TRC) method, which is a PCR-based technique producing more stable and reliable results, because it is a more simplified process compared with the conventional techniques, has been introduced for detecting micrometastasis in some carcinomas. We aimed to demonstrate the effectiveness of TRC method in the CTC detection. Methods. We compared the two methods for the sensitivity for CTC detection using the colon cancer cell line and 42 whole-blood samples from patients with advanced or metastatic CRC. Furthermore, 25 patients with metastatic CRC were enrolled to investigate the correlation between CTC detection and prognosis in both methods. Results. The sensitivity of the TRC method was similar to that of the CellSearch system. The overall survival rate was significantly worse in the patients diagnosed as CTCpositive by the TRC method than in those diagnosed as CTC-negative; this finding was similar to the prognosis indicated by the CellSearch system. However, clinically, the TRC method could detect CTCs more rapidly and at a reduced cost compared with the CellSearch system. Conclusions. The TRC method seems to be a useful alternative to the CellSearch system for clinically detecting CTCs in patients with metastatic CRC.
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U2 - 10.1245/s10434-011-1889-7
DO - 10.1245/s10434-011-1889-7
M3 - Article
C2 - 21732137
AN - SCOPUS:84865135059
SN - 1068-9265
VL - 19
SP - 2060
EP - 2065
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 6
ER -
