Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer

Nobutaka Sato, Naoko Hayashi, Yu Imamura, Yohei Tanaka, Koichi Kinoshita, Junji Kurashige, Seiya Saito, Ryuichi Karashima, Kotaro Hirashima, Yohei Nagai, Yuji Miyamoto, Masaaki Iwatsuki, Yoshifumi Baba, Masayuki Watanabe, Hideo Baba

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose. The CellSearch system (Veridex, LLC) is useful for detecting circulating tumor cells (CTCs) in various carcinomas, including colorectal cancer (CRC); however, there are some problems associated with its clinical use. A transcription-reverse transcription concerted reaction (TRC) method, which is a PCR-based technique producing more stable and reliable results, because it is a more simplified process compared with the conventional techniques, has been introduced for detecting micrometastasis in some carcinomas. We aimed to demonstrate the effectiveness of TRC method in the CTC detection. Methods. We compared the two methods for the sensitivity for CTC detection using the colon cancer cell line and 42 whole-blood samples from patients with advanced or metastatic CRC. Furthermore, 25 patients with metastatic CRC were enrolled to investigate the correlation between CTC detection and prognosis in both methods. Results. The sensitivity of the TRC method was similar to that of the CellSearch system. The overall survival rate was significantly worse in the patients diagnosed as CTCpositive by the TRC method than in those diagnosed as CTC-negative; this finding was similar to the prognosis indicated by the CellSearch system. However, clinically, the TRC method could detect CTCs more rapidly and at a reduced cost compared with the CellSearch system. Conclusions. The TRC method seems to be a useful alternative to the CellSearch system for clinically detecting CTCs in patients with metastatic CRC.

Original languageEnglish
Pages (from-to)2060-2065
Number of pages6
JournalAnnals of Surgical Oncology
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 1 2012

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Circulating Neoplastic Cells
Reverse Transcription
Colorectal Neoplasms
Carcinoma
Neoplasm Micrometastasis
Colonic Neoplasms
Survival Rate
Costs and Cost Analysis
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer. / Sato, Nobutaka; Hayashi, Naoko; Imamura, Yu; Tanaka, Yohei; Kinoshita, Koichi; Kurashige, Junji; Saito, Seiya; Karashima, Ryuichi; Hirashima, Kotaro; Nagai, Yohei; Miyamoto, Yuji; Iwatsuki, Masaaki; Baba, Yoshifumi; Watanabe, Masayuki; Baba, Hideo.

In: Annals of Surgical Oncology, Vol. 19, No. 6, 01.06.2012, p. 2060-2065.

Research output: Contribution to journalArticle

Sato, N, Hayashi, N, Imamura, Y, Tanaka, Y, Kinoshita, K, Kurashige, J, Saito, S, Karashima, R, Hirashima, K, Nagai, Y, Miyamoto, Y, Iwatsuki, M, Baba, Y, Watanabe, M & Baba, H 2012, 'Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer', Annals of Surgical Oncology, vol. 19, no. 6, pp. 2060-2065. https://doi.org/10.1245/s10434-011-1889-7
Sato, Nobutaka ; Hayashi, Naoko ; Imamura, Yu ; Tanaka, Yohei ; Kinoshita, Koichi ; Kurashige, Junji ; Saito, Seiya ; Karashima, Ryuichi ; Hirashima, Kotaro ; Nagai, Yohei ; Miyamoto, Yuji ; Iwatsuki, Masaaki ; Baba, Yoshifumi ; Watanabe, Masayuki ; Baba, Hideo. / Usefulness of transcription-reverse transcription concerted reaction method for detecting circulating tumor cells in patients with colorectal cancer. In: Annals of Surgical Oncology. 2012 ; Vol. 19, No. 6. pp. 2060-2065.
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AU - Sato, Nobutaka

AU - Hayashi, Naoko

AU - Imamura, Yu

AU - Tanaka, Yohei

AU - Kinoshita, Koichi

AU - Kurashige, Junji

AU - Saito, Seiya

AU - Karashima, Ryuichi

AU - Hirashima, Kotaro

AU - Nagai, Yohei

AU - Miyamoto, Yuji

AU - Iwatsuki, Masaaki

AU - Baba, Yoshifumi

AU - Watanabe, Masayuki

AU - Baba, Hideo

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