Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video)

Masaya Suenaga, Yoshihiko Sadakari, Jose Alejandro Almario, Michael Borges, Anne Marie Lennon, Eun Ji Shin, Marcia Irene Canto, Michael Goggins

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and Aims Duodenal collections of pancreatic fluid can be used as a source of mutations and other markers of pancreatic ductal neoplasia, but admixing pancreatic juice with duodenal contents lowers the concentrations of mutations. Collecting pancreatic fluid directly from the ampulla could yield a purer sample of pancreatic fluid. Methods We used an endoscopic distal cap attachment to “cap” the ampulla and collect secretin-stimulated pancreatic fluid samples for 5 minutes from 81 patients undergoing pancreatic evaluation as part of the Cancer of the Pancreas Screening studies. We compared mutation concentrations (K-ras and GNAS) measured by droplet-digital PCR (ddPCR) in “cap-collected juice” samples to those found in juice samples obtained from 77 patients collected by aspiration from the duodenal lumen without capping the ampulla. Results Among all subjects, mutation concentrations were higher in pancreatic juice samples collected using the endoscopic cap method (median,.028%; IQR, 0-.077) compared with the noncap-collected (median,.019%; IQR, 0-.044; P =.055). Among pancreatic juice samples with detectable mutations, mutation concentrations were higher in the cap-collected juice samples than in those collected without the cap (.055%; IQR,.026-.092 vs.032%; IQR,.020-.066; P =.031). Conclusions Collecting pancreatic juice directly from the ampulla using an endoscopic distal cap yields higher concentrations of pancreatic fluid mutations.

Original languageEnglish
Pages (from-to)1152-1156.e2
JournalGastrointestinal endoscopy
Volume86
Issue number6
DOIs
Publication statusPublished - Dec 2017

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Pancreatic Juice
Mutation
Secretin
Pancreatic Neoplasms
Early Detection of Cancer
Polymerase Chain Reaction
Neoplasms

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Gastroenterology

Cite this

Suenaga, M., Sadakari, Y., Almario, J. A., Borges, M., Lennon, A. M., Shin, E. J., ... Goggins, M. (2017). Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video). Gastrointestinal endoscopy, 86(6), 1152-1156.e2. https://doi.org/10.1016/j.gie.2017.02.026

Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video). / Suenaga, Masaya; Sadakari, Yoshihiko; Almario, Jose Alejandro; Borges, Michael; Lennon, Anne Marie; Shin, Eun Ji; Canto, Marcia Irene; Goggins, Michael.

In: Gastrointestinal endoscopy, Vol. 86, No. 6, 12.2017, p. 1152-1156.e2.

Research output: Contribution to journalArticle

Suenaga, M, Sadakari, Y, Almario, JA, Borges, M, Lennon, AM, Shin, EJ, Canto, MI & Goggins, M 2017, 'Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video)', Gastrointestinal endoscopy, vol. 86, no. 6, pp. 1152-1156.e2. https://doi.org/10.1016/j.gie.2017.02.026
Suenaga M, Sadakari Y, Almario JA, Borges M, Lennon AM, Shin EJ et al. Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video). Gastrointestinal endoscopy. 2017 Dec;86(6):1152-1156.e2. https://doi.org/10.1016/j.gie.2017.02.026
Suenaga, Masaya ; Sadakari, Yoshihiko ; Almario, Jose Alejandro ; Borges, Michael ; Lennon, Anne Marie ; Shin, Eun Ji ; Canto, Marcia Irene ; Goggins, Michael. / Using an endoscopic distal cap to collect pancreatic fluid from the ampulla (with video). In: Gastrointestinal endoscopy. 2017 ; Vol. 86, No. 6. pp. 1152-1156.e2.
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