Utilization of central disk of blastoderm and germinal crescent region for production of interspecific germline chimera between chicken and quail

Tomoki Soh, Yoshie Inoue, Yong Mei Xi, Yukio Kato, Masa Aki Hattori

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Aim: The production of interspecific germline chimeras between chicken and quail were attempted employing the dissociated cells derived from the blastodermal central disk (stage X) and the germinal crescent region of embryo (stage 7-8). Methods: The central disk (CD) of the area pellucida in chicken blastoderm (stage X) and the germinal crescent region (GCR) of embryo (stage 7-8) were dispersed and injected into the subgerminal cavity of quail blastoderm (stage X). Injected eggs were incubated for 7 days or to hatching. The donor chicken DNA was detected by the polymerase chain reaction. Results: In day-7 embryos, chicken DNA was detected in 5 gonads and 9 brains from 53 survived embryos received chicken CD cells, and 1 gonads and 6 brains from 27 survived embryos received chicken GCR. Chicken DNA was also detected from the semen of one adult male hatched from eggs received chicken GCR cells. Conclusion: CD and GCR cells as the donors showed the possibility to produce the interspecific germline chimera, but further studies are needed to make necessary improvement.

Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalAsian Journal of Andrology
Volume4
Issue number2
Publication statusPublished - Jan 1 2002

All Science Journal Classification (ASJC) codes

  • Urology

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