UV-B-induced CPD photolyase gene expression is regulated by UVR8-dependent and-independent pathways in arabidopsis

Nan Li, Mika Teranishi, Hiroko Yamaguchi, Tomonao Matsushita, Masaaki K. Watahiki, Tomohiko Tsuge, Shao Shan Li, Jun Hidema

Research output: Contribution to journalArticle

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Abstract

Plants have evolved various mechanisms that protect against the harmful effects of UV-B radiation (280-315 nm) on growth and development. Cyclobutane pyrimidine dimer (CPD) photolyase, the repair enzyme for UV-B-induced CPDs, is essential for protecting cells from UV-B radiation. Expression of the CPD photolyase gene (PHR) is controlled by light with various wavelengths including UV-B, but the mechanisms of this regulation remain poorly understood. In this study, we investigated the regulation of PHR expression by light with various wavelengths, in particular low-fluence UV-B radiation (280 nm, 0.2 μmol m-2 s-1), in Arabidopsis thaliana seedlings grown under light-dark cycles for 7 d and then adapted to the dark for 3 d. Low-fluence UV-B radiation induced CPDs but not reactive oxygen species. AtPHR expression was effectively induced by UV-B, UV-A (375 nm) and blue light. Expression induced by UV-A and blue light was predominantly regulated by the cryptochrome-dependent pathway, whereas phytochromes A and B played a minor but noticeable role. Expression induced by UV-B was predominantly regulated by the UVR8-dependent pathway. AtPHR expression was also mediated by a UVR8-independent pathway, which is correlated with CPD accumulation induced by UV-B radiation. These results indicate that Arabidopsis has evolved diverse mechanisms to regulate CPD photolyase expression by multiple photoreceptor signaling pathways, including UVR8-dependent and -independent pathways, as protection against harmful effects of UV-B radiation.

Original languageEnglish
Pages (from-to)2014-2023
Number of pages10
JournalPlant and Cell Physiology
Volume56
Issue number10
DOIs
Publication statusPublished - Jan 8 2015

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Deoxyribodipyrimidine Photo-Lyase
Pyrimidine Dimers
pyrimidines
Arabidopsis
ultraviolet radiation
Radiation
Gene Expression
gene expression
Light
Phytochrome B
Phytochrome A
Cryptochromes
blue light
Photoperiod
wavelengths
Seedlings
Growth and Development
Reactive Oxygen Species
phytochrome
photoreceptors

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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UV-B-induced CPD photolyase gene expression is regulated by UVR8-dependent and-independent pathways in arabidopsis. / Li, Nan; Teranishi, Mika; Yamaguchi, Hiroko; Matsushita, Tomonao; Watahiki, Masaaki K.; Tsuge, Tomohiko; Li, Shao Shan; Hidema, Jun.

In: Plant and Cell Physiology, Vol. 56, No. 10, 08.01.2015, p. 2014-2023.

Research output: Contribution to journalArticle

Li, Nan ; Teranishi, Mika ; Yamaguchi, Hiroko ; Matsushita, Tomonao ; Watahiki, Masaaki K. ; Tsuge, Tomohiko ; Li, Shao Shan ; Hidema, Jun. / UV-B-induced CPD photolyase gene expression is regulated by UVR8-dependent and-independent pathways in arabidopsis. In: Plant and Cell Physiology. 2015 ; Vol. 56, No. 10. pp. 2014-2023.
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