Vδ1 + γδ T cells producing CC chemokines may bridge a gap between neutrophils and macrophages in innate immunity during Escherichia coli infection in mice

Tetsuzo Tagawa, Hitoshi Nishimura, Toshiki Yajima, Hiromitsu Hara, Kenji Kishihara, Goro Matsuzaki, Ichiro Yoshino, Yoshihiko Maehara, Yasunobu Yoshikai

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Abstract

An influx of neutrophils followed a short time later by an influx of macrophages to the infected site plays a key role in innate immunity against Escherichia coli infection. We found in this study that Vδ1 -/- mice exhibited impaired accumulation of peritoneal macrophages but not neutrophils and delayed bacterial clearance after i.p. inoculation with E. coli. Peritoneal γδ T cells from E. coli-infected wild-type mice produced CCL3/MIP-1α and CCL5/RANTES in response to γδ TCR triggering in vitro, whereas such production was not evident in γδ T cells from E. coli-infected Vδ1 -/- mice. Neutralization of CCL3/MIP-1α by a specific mAb in vivo significantly inhibited the accumulation of macrophages in the peritoneal cavity after E. coli infection, resulting in exacerbated bacterial growth in the peritoneal cavity. These results suggest that Vδ1 + γδ T cells bridge a gap between neutrophils and macrophages in innate immunity during E. coli infection mediated by production of CC chemokines, enhancing macrophage trafficking to the site of infection.

Original languageEnglish
Pages (from-to)5156-5164
Number of pages9
JournalJournal of Immunology
Volume173
Issue number8
Publication statusPublished - Oct 15 2004

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Escherichia coli Infections
CC Chemokines
Innate Immunity
Neutrophils
Macrophages
T-Lymphocytes
Peritoneal Cavity
Escherichia coli
Chemokine CCL5
Peritoneal Macrophages
Growth
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Vδ1 + γδ T cells producing CC chemokines may bridge a gap between neutrophils and macrophages in innate immunity during Escherichia coli infection in mice. / Tagawa, Tetsuzo; Nishimura, Hitoshi; Yajima, Toshiki; Hara, Hiromitsu; Kishihara, Kenji; Matsuzaki, Goro; Yoshino, Ichiro; Maehara, Yoshihiko; Yoshikai, Yasunobu.

In: Journal of Immunology, Vol. 173, No. 8, 15.10.2004, p. 5156-5164.

Research output: Contribution to journalArticle

Tagawa, T, Nishimura, H, Yajima, T, Hara, H, Kishihara, K, Matsuzaki, G, Yoshino, I, Maehara, Y & Yoshikai, Y 2004, 'Vδ1 + γδ T cells producing CC chemokines may bridge a gap between neutrophils and macrophages in innate immunity during Escherichia coli infection in mice', Journal of Immunology, vol. 173, no. 8, pp. 5156-5164.
Tagawa, Tetsuzo ; Nishimura, Hitoshi ; Yajima, Toshiki ; Hara, Hiromitsu ; Kishihara, Kenji ; Matsuzaki, Goro ; Yoshino, Ichiro ; Maehara, Yoshihiko ; Yoshikai, Yasunobu. / Vδ1 + γδ T cells producing CC chemokines may bridge a gap between neutrophils and macrophages in innate immunity during Escherichia coli infection in mice. In: Journal of Immunology. 2004 ; Vol. 173, No. 8. pp. 5156-5164.
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