Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity

N. Kobayashi, G. Matsuzaki, Yasunobu Yoshikai, R. Seki, J. Ivanyi, K. Nomoto

    Research output: Contribution to journalArticle

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    Abstract

    The purpose of this paper was to study the γδ T-cell receptor repertoire and target specificity following stimulation of peripheral T cells of BALB/c mice with autologous or bacterial ligands. The expression of Vγ and Vδ chain families in T cells that had been expanded by stimulation in syngeneic mixed lymphocyte culture or with purified protein derivative (PPD) was determined by the semiquantitative DNA polymerase chain reaction (PCR) method. Responder T cells to either of these stimuli strongly expressed both Vδ5 and Vδ6 genes. However, addition of the ML 30 anti-murine heat shock protein (hsp) 60 monoclonal antibody (mAb) to the cell culture selectively inhibited only the expansion of Vδ5 T cells. A Vδ5 T-cell hybridoma (KMT- 5), which recognized syngeneic splenic and fibrosarcoma Meth A cells but not allogeneic cells, was produced by cell fusion from autoreactive blast cells. Incubation of the KMT-5 hybridoma in the presence of ML 30 antibody blocked the stimulation of interleukin-2 (IL-2) secretion by syngeneic target cells. It was also found that the DNA of KMT-5 hybridoma and of the autoreactive γδ T cells contained the BALB/c invariant delta (BID) chain sequence. It is concluded from these results that BALB/c peripheral Vδ5 T cells recognize an autologous hsp 60 target specificity in a Vδ-gene restricted manner. We also propose that T cells of this V gene family may be involved in the immune surveillance of certain tumours and intracellular infections.

    Original languageEnglish
    Pages (from-to)240-246
    Number of pages7
    JournalImmunology
    Volume81
    Issue number2
    Publication statusPublished - Jan 1 1994

    Fingerprint

    Chaperonin 60
    T-Lymphocytes
    Hybridomas
    Genes
    Cell Fusion
    Fibrosarcoma
    DNA-Directed DNA Polymerase
    T-Cell Antigen Receptor
    Interleukin-2
    Cell Culture Techniques
    Monoclonal Antibodies
    Lymphocytes
    Ligands
    Polymerase Chain Reaction
    Antibodies
    DNA

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Kobayashi, N., Matsuzaki, G., Yoshikai, Y., Seki, R., Ivanyi, J., & Nomoto, K. (1994). Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity. Immunology, 81(2), 240-246.

    Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity. / Kobayashi, N.; Matsuzaki, G.; Yoshikai, Yasunobu; Seki, R.; Ivanyi, J.; Nomoto, K.

    In: Immunology, Vol. 81, No. 2, 01.01.1994, p. 240-246.

    Research output: Contribution to journalArticle

    Kobayashi, N, Matsuzaki, G, Yoshikai, Y, Seki, R, Ivanyi, J & Nomoto, K 1994, 'Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity', Immunology, vol. 81, no. 2, pp. 240-246.
    Kobayashi N, Matsuzaki G, Yoshikai Y, Seki R, Ivanyi J, Nomoto K. Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity. Immunology. 1994 Jan 1;81(2):240-246.
    Kobayashi, N. ; Matsuzaki, G. ; Yoshikai, Yasunobu ; Seki, R. ; Ivanyi, J. ; Nomoto, K. / Vδ5+ T cells of BALB/c mice recognize the murine heat shock protein 60 target cell specificity. In: Immunology. 1994 ; Vol. 81, No. 2. pp. 240-246.
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