V domain of human SLAM (CDw150) is essential for its function as a measles virus receptor

N. Ono, H. Tatsuo, K. Tanaka, H. Minagawa, Yusuke Yanagi

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Human signaling lymphocytic activation molecule (SLAM; also known as CDw150) has been shown to be a cellular receptor for measles virus (MV). Chinese hamster ovary cells transfected with a mouse SLAM cDNA were not susceptible to MV and the vesicular stomatitis virus pseudotype bearing MV envelope proteins alone, indicating that mouse SLAM cannot act as an MV receptor. To determine the functional domain of the receptor, we tested the abilities of several chimeric SLAM proteins to function as MV receptors. The ectodomain of SLAM comprises the two immunoglobulin superfamily domains (V and C2). Various chimeric transmembrane proteins possessing the V domain of human SLAM were able to act as MV receptors, whereas a chimera consisting of human SLAM containing the mouse V domain instead of the human V domain no longer acted as a receptor. To examine the interaction between SLAM and MV envelope proteins, recombinant soluble forms of SLAM were produced. The soluble molecules possessing the V domain of human SLAM were shown to bind to cells expressing the MV hemagglutinin (H) protein but not to cells expressing the MV fusion protein or irrelevant envelope proteins. These results indicate that the V domain of human SLAM is necessary and sufficient to interact with the MV H protein and allow MV entry.

Original languageEnglish
Pages (from-to)1594-1600
Number of pages7
JournalJournal of Virology
Volume75
Issue number4
DOIs
Publication statusPublished - Feb 12 2001

Fingerprint

Measles virus
Virus Receptors
Viral Fusion Proteins
Viral Envelope Proteins
recombinant fusion proteins
receptors
mice
virus receptors
viral fusion proteins
proteins
Virus Internalization
Vesicular Stomatitis
Vesiculovirus
Hemagglutinins
Cricetulus
transmembrane proteins
chimerism
Chinese hamsters
cells
hemagglutinins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

V domain of human SLAM (CDw150) is essential for its function as a measles virus receptor. / Ono, N.; Tatsuo, H.; Tanaka, K.; Minagawa, H.; Yanagi, Yusuke.

In: Journal of Virology, Vol. 75, No. 4, 12.02.2001, p. 1594-1600.

Research output: Contribution to journalArticle

Ono, N. ; Tatsuo, H. ; Tanaka, K. ; Minagawa, H. ; Yanagi, Yusuke. / V domain of human SLAM (CDw150) is essential for its function as a measles virus receptor. In: Journal of Virology. 2001 ; Vol. 75, No. 4. pp. 1594-1600.
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