Valosin-containing protein/p97 interacts with sperm-activating and sperm-attracting factor (SAAF) in the ascidian egg and modulates sperm-attracting activity

Eri Kondoh, Aru Konno, Kazuo Inaba, Tohru Oishi, Michio Murata, Manabu Yoshida

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Sperm chemotaxis toward an egg is observed in many animals, and the control of sperm-attracting activity is thought to play an important role in ensuring fertilization. However, the mechanism underlying the release of a sperm attractant from an egg is still obscure. In this study, we examined the systems involved in the release of sperm-activating and sperm-attracting factor (SAAF), which is the sperm attractant of the ascidian Ciona intestinalis. Here, we show that the egg acquires sperm-attracting activity after germinal vesicle breakdown. Further, since the cytoplasmic extracts of immature oocytes exhibit no sperm-attracting activity, the SAAF in oocytes may be activated after germinal vesicle breakdown. We found 13 SAAF-binding proteins in an egg plasma membrane extract and identified five proteins by proteomic analysis: valosin-containing protein (VCP)/p97, proteasome alpha 2 subunit, MGC97756 protein, proteasome subunit Y, and beta-tubulin. In particular, the interaction between VCP/p97 and SAAF was confirmed by a pull-down assay. VCP/p97 is initially localized in the germinal vesicle, and during oocyte maturation, it shifts to the endoplasmic reticulum in the cortical regions. Thus, VCP/p97 is a potential modulator of SAAF release from the egg.

Original languageEnglish
Pages (from-to)665-673
Number of pages9
JournalDevelopment Growth and Differentiation
Volume50
Issue number8
DOIs
Publication statusPublished - Oct 1 2008
Externally publishedYes

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Urochordata
Ovum
Spermatozoa
Oocytes
Proteasome Endopeptidase Complex
CDC48 protein
Ciona intestinalis
Protein Subunits
Chemotaxis
Tubulin
Fertilization
Endoplasmic Reticulum
Proteomics
Carrier Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Valosin-containing protein/p97 interacts with sperm-activating and sperm-attracting factor (SAAF) in the ascidian egg and modulates sperm-attracting activity. / Kondoh, Eri; Konno, Aru; Inaba, Kazuo; Oishi, Tohru; Murata, Michio; Yoshida, Manabu.

In: Development Growth and Differentiation, Vol. 50, No. 8, 01.10.2008, p. 665-673.

Research output: Contribution to journalArticle

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AU - Konno, Aru

AU - Inaba, Kazuo

AU - Oishi, Tohru

AU - Murata, Michio

AU - Yoshida, Manabu

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