Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc

Ken Takai, Takuro Nunoura, Koki Horikoshi, Takazo Shibuya, Kentaro Nakamura, Yohey Suzuki, Matthew Stott, Gary J. Massoth, B. W. Christenson, Cornel E.J. deRonde, David A. Butterfield, Junichiro Ishibashi, John E. Lupton, L. J. Evans

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.

Original languageEnglish
Pages (from-to)252-269
Number of pages18
JournalGeomicrobiology Journal
Volume26
Issue number8
DOIs
Publication statusPublished - Dec 1 2009

Fingerprint

black smoker
Chimneys
Volcanoes
Vents
caldera
rRNA Genes
Hydrothermal Vents
Siblings
microbial community
volcano
Gases
Archaeal Genes
Bacterial Genes
Salinity
Fluids
Oceans and Seas
hydrothermal fluid
Carbon
Clone Cells
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

Cite this

Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc. / Takai, Ken; Nunoura, Takuro; Horikoshi, Koki; Shibuya, Takazo; Nakamura, Kentaro; Suzuki, Yohey; Stott, Matthew; Massoth, Gary J.; Christenson, B. W.; deRonde, Cornel E.J.; Butterfield, David A.; Ishibashi, Junichiro; Lupton, John E.; Evans, L. J.

In: Geomicrobiology Journal, Vol. 26, No. 8, 01.12.2009, p. 252-269.

Research output: Contribution to journalArticle

Takai, K, Nunoura, T, Horikoshi, K, Shibuya, T, Nakamura, K, Suzuki, Y, Stott, M, Massoth, GJ, Christenson, BW, deRonde, CEJ, Butterfield, DA, Ishibashi, J, Lupton, JE & Evans, LJ 2009, 'Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc', Geomicrobiology Journal, vol. 26, no. 8, pp. 252-269. https://doi.org/10.1080/01490450903304949
Takai, Ken ; Nunoura, Takuro ; Horikoshi, Koki ; Shibuya, Takazo ; Nakamura, Kentaro ; Suzuki, Yohey ; Stott, Matthew ; Massoth, Gary J. ; Christenson, B. W. ; deRonde, Cornel E.J. ; Butterfield, David A. ; Ishibashi, Junichiro ; Lupton, John E. ; Evans, L. J. / Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc. In: Geomicrobiology Journal. 2009 ; Vol. 26, No. 8. pp. 252-269.
@article{8903fca146704c8ea55f4ad756be7d02,
title = "Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc",
abstract = "Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.",
author = "Ken Takai and Takuro Nunoura and Koki Horikoshi and Takazo Shibuya and Kentaro Nakamura and Yohey Suzuki and Matthew Stott and Massoth, {Gary J.} and Christenson, {B. W.} and deRonde, {Cornel E.J.} and Butterfield, {David A.} and Junichiro Ishibashi and Lupton, {John E.} and Evans, {L. J.}",
year = "2009",
month = "12",
day = "1",
doi = "10.1080/01490450903304949",
language = "English",
volume = "26",
pages = "252--269",
journal = "Geomicrobiology Journal",
issn = "0149-0451",
publisher = "Taylor and Francis Ltd.",
number = "8",

}

TY - JOUR

T1 - Variability in microbial communities in black smoker chimneys at the NW caldera vent field, brothers volcano, Kermadec arc

AU - Takai, Ken

AU - Nunoura, Takuro

AU - Horikoshi, Koki

AU - Shibuya, Takazo

AU - Nakamura, Kentaro

AU - Suzuki, Yohey

AU - Stott, Matthew

AU - Massoth, Gary J.

AU - Christenson, B. W.

AU - deRonde, Cornel E.J.

AU - Butterfield, David A.

AU - Ishibashi, Junichiro

AU - Lupton, John E.

AU - Evans, L. J.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.

AB - Microbial communities in black smoker chimney structures at the NW caldera vent field of the Brothers volcano, Kermadec arc were characterized by using both culture-dependent and - independent techniques. The hydrothermal vent fluid chemistry, as given by end-member salinities and gas contents, differ among the black smoker sites of the NW caldera field, indicating probable phase-separation-controlled variability in the fluid chemistry. Chimney structures collected from typical Cl-depleted and Clenriched hydrothermal fluid vents were used for the microbiological investigation. The 16S rRNA gene clone analysis showed that the archaeal rRNA gene communities were similar within interior and exterior substructures of any single chimneys, and even between chimneys having either Cl-depleted or Cl-enriched hydrothermal emissions. By contrast, the bacterial rRNA gene communities varied between chimneys hosting Cl-depleted or Clenriched fluids. Cultivation analysis showed significant variation in the viable counts of various microbial components among the chimneys, particularly of H2 - and/or S-oxidizing chemolithotrophs such as the genera Persephonella and Sulfurimonas. The difference shown by the cultured microbial community structures between the chimneys may be related to the different chemistries of hydrothermal fluids being expelled by the chimney structures, and possibly differences in the subseafloor environments beneath the vent sites, especially when considering different gas inputs and carbon sources. The patterns in cultivated microbial populations in the chimney structures were compared among the chimney structures studied so far from various deep-sea hydrothermal fields including this study. It shows that the patterns from the gas-rich hydrothermal fluid chimneys are quite similar between the geographically and geologically different hydrothermal fields of the Brothers NW caldera vent field, and the Mariner vent field of the Valu Fa Ridge.

UR - http://www.scopus.com/inward/record.url?scp=71149094725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71149094725&partnerID=8YFLogxK

U2 - 10.1080/01490450903304949

DO - 10.1080/01490450903304949

M3 - Article

VL - 26

SP - 252

EP - 269

JO - Geomicrobiology Journal

JF - Geomicrobiology Journal

SN - 0149-0451

IS - 8

ER -