Abstract
This letter describes the spectroscopic measurements of fluorescently stained cells. Variable phase-contrast fluorescence spectrometry was used on fluorescently stained cells to achieve high two-dimensional spatial resolution. Phase shift interferometry by autocorrelation interference made it possible to measure fluorescence spectra in the field of view without the separation of wavelengths, as in the case of a conventional dispersive spectrometer. In this letter, the authors describe the experimental characteristics of fluorescence spectra generated from fluorescently stained cells and verify that the fluorescence spectra of the stained area in the cells can be measured by our method.
| Original language | English |
|---|---|
| Article number | 121103 |
| Journal | Applied Physics Letters |
| Volume | 89 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Sep 29 2006 |
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All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)
Cite this
Variable phase-contrast fluorescence spectrometry for fluorescently stained cells. / Inoue, Yusuke; Ishimaru, Ichirou; Yasokawa, Toshiki; Ishizaki, Katsumi; Yoshida, Makoto; Kondo, Masahiko; Kuriyama, Shigeki; Masaki, Tsutomu; Nakai, Seiji; Takegawa, Kaoru; Tanaka, Naotaka.
In: Applied Physics Letters, Vol. 89, No. 12, 121103, 29.09.2006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Variable phase-contrast fluorescence spectrometry for fluorescently stained cells
AU - Inoue, Yusuke
AU - Ishimaru, Ichirou
AU - Yasokawa, Toshiki
AU - Ishizaki, Katsumi
AU - Yoshida, Makoto
AU - Kondo, Masahiko
AU - Kuriyama, Shigeki
AU - Masaki, Tsutomu
AU - Nakai, Seiji
AU - Takegawa, Kaoru
AU - Tanaka, Naotaka
PY - 2006/9/29
Y1 - 2006/9/29
N2 - This letter describes the spectroscopic measurements of fluorescently stained cells. Variable phase-contrast fluorescence spectrometry was used on fluorescently stained cells to achieve high two-dimensional spatial resolution. Phase shift interferometry by autocorrelation interference made it possible to measure fluorescence spectra in the field of view without the separation of wavelengths, as in the case of a conventional dispersive spectrometer. In this letter, the authors describe the experimental characteristics of fluorescence spectra generated from fluorescently stained cells and verify that the fluorescence spectra of the stained area in the cells can be measured by our method.
AB - This letter describes the spectroscopic measurements of fluorescently stained cells. Variable phase-contrast fluorescence spectrometry was used on fluorescently stained cells to achieve high two-dimensional spatial resolution. Phase shift interferometry by autocorrelation interference made it possible to measure fluorescence spectra in the field of view without the separation of wavelengths, as in the case of a conventional dispersive spectrometer. In this letter, the authors describe the experimental characteristics of fluorescence spectra generated from fluorescently stained cells and verify that the fluorescence spectra of the stained area in the cells can be measured by our method.
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UR - http://www.scopus.com/inward/citedby.url?scp=33748975526&partnerID=8YFLogxK
U2 - 10.1063/1.2356312
DO - 10.1063/1.2356312
M3 - Article
VL - 89
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 12
M1 - 121103
ER -