Variable phase-contrast fluorescence spectrometry for fluorescently stained cells

Yusuke Inoue, Ichirou Ishimaru, Toshiki Yasokawa, Katsumi Ishizaki, Makoto Yoshida, Masahiko Kondo, Shigeki Kuriyama, Tsutomu Masaki, Seiji Nakai, Kaoru Takegawa, Naotaka Tanaka

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Abstract

This letter describes the spectroscopic measurements of fluorescently stained cells. Variable phase-contrast fluorescence spectrometry was used on fluorescently stained cells to achieve high two-dimensional spatial resolution. Phase shift interferometry by autocorrelation interference made it possible to measure fluorescence spectra in the field of view without the separation of wavelengths, as in the case of a conventional dispersive spectrometer. In this letter, the authors describe the experimental characteristics of fluorescence spectra generated from fluorescently stained cells and verify that the fluorescence spectra of the stained area in the cells can be measured by our method.

Original languageEnglish
Article number121103
JournalApplied Physics Letters
Volume89
Issue number12
DOIs
Publication statusPublished - Sep 29 2006
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Inoue, Y., Ishimaru, I., Yasokawa, T., Ishizaki, K., Yoshida, M., Kondo, M., ... Tanaka, N. (2006). Variable phase-contrast fluorescence spectrometry for fluorescently stained cells. Applied Physics Letters, 89(12), [121103]. https://doi.org/10.1063/1.2356312