Variation in Hematopoietic Potential of Induced Pluripotent Stem Cell Lines

Kasem Kulkeaw, Yuka Horio, Chiyo Mizuochi, Minetaro Ogawa, Daisuke Sugiyama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells were originally generated from somatic cells by ectopic expression of four transcription factor genes: Oct3/4, Sox2, Klf4 and c-Myc. Currently, iPS cell lines differ in tissue origin, the combination of factors used to construct them, the method of gene delivery and expression of pluripotency markers. Thus to evaluate iPS cells for haematotherapy, the hematopoietic potential among iPS lines should be compared. Here, we compare differentiation capacity of six iPS lines into mesodermal cells and hematopoietic cells (HCs) through embryoid body (EB) formation. We show that the mouse embryonic fibroblast (MEF)-derived iPS lines 20D17 and 178B5 resemble CCE ES cells in terms of morphology in culture, number and size of EBs and differentiation capacity into mesodermal cells compared to iPS cells derived from adults, although all iPS lines could form EBs. The number of mesodermal cells differentiated from MEF-derived iPS cell lines showed a 3.9-407-fold increase compared to that from iPS lines derived from adults. Furthermore, 178B5 iPS cells generated Ter119+ erythroid cells (3.35%) efficiently in culture. We conclude that hematopoietic potential differs among the six lines and that MEF-derived 20D17 and 178B5 iPS cells generate HCs more efficiently than adult-derived iPS cells.

Original languageEnglish
Pages (from-to)381-389
Number of pages9
JournalStem Cell Reviews and Reports
Volume6
Issue number3
DOIs
Publication statusPublished - Apr 16 2010

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Induced Pluripotent Stem Cells
Cell Line
Fibroblasts
Embryoid Bodies
Erythroid Cells
Transcription Factors
Cell Count
Gene Expression

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Cancer Research

Cite this

Variation in Hematopoietic Potential of Induced Pluripotent Stem Cell Lines. / Kulkeaw, Kasem; Horio, Yuka; Mizuochi, Chiyo; Ogawa, Minetaro; Sugiyama, Daisuke.

In: Stem Cell Reviews and Reports, Vol. 6, No. 3, 16.04.2010, p. 381-389.

Research output: Contribution to journalArticle

Kulkeaw, Kasem ; Horio, Yuka ; Mizuochi, Chiyo ; Ogawa, Minetaro ; Sugiyama, Daisuke. / Variation in Hematopoietic Potential of Induced Pluripotent Stem Cell Lines. In: Stem Cell Reviews and Reports. 2010 ; Vol. 6, No. 3. pp. 381-389.
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