Variations in the expression of photosynthesis-related proteins in field Chattonella marina cells during a harmful algal bloom

Xuchun Qiu, Kokl Mukai, Yohei Shimasaki, Michito Tsuyama, Tadashi Matsubara, Takashi Nakashima, Hirofumi Ichinose, Yu Nakajima, Ibuneo Honjo, Yuji Oshima

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Abstract

Time series variations in protein expression profiles in field Chattonella marina cells were investigated during a HAB occurred in the inner part of Ariake Sea, Japan (5-14 September, 2012). This study aimed to gather information on the molecular mechanisms underlying the physiological responses in field C. marina population during HAB. Proteomic analysis showed that the abundance of -37% protein spots (40 out of 108 detected from 2-DE gel images) significantly varied with the sampling date. Significant decreases in the abundances of proteins involved in photosystem II (LHCP 4), electron transfer chain (Cyt c553), Calvin cycle (GAPDH), and chloroplast antioxidant system (2-Cys Prx) were observed as the bloom progressed, suggesting the efficiencies of those photosynthetic pathways declined during the bloom. In addition, the abundances of the above proteins showed significant positive correlations with the Fv/Fm ratio and growth rate of C. marina and with DIN concentrations (except LHCP 4). Our findings suggested that declined expressions of those photosynthesis-related proteins presented some molecular foundation of the decreases in Fv/Fm ratio and growth rate of C. marina during the bloom, and also provided insight into mechanistic links between the external/internal factors and physiological responses of C. marina that may ultimately dictate the ecology of the bloom.

Original languageEnglish
Pages (from-to)373-380
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume62
Issue number2
Publication statusPublished - Sep 1 2017

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Harmful Algal Bloom
Photosynthesis
algal blooms
photosynthesis
physiological response
Proteins
proteins
cells
Calvin cycle
electron transport chain
Photosystem II Protein Complex
proteomics
photosystem II
time series analysis
Chloroplasts
Growth
Ecology
chloroplasts
Oceans and Seas
protein synthesis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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title = "Variations in the expression of photosynthesis-related proteins in field Chattonella marina cells during a harmful algal bloom",
abstract = "Time series variations in protein expression profiles in field Chattonella marina cells were investigated during a HAB occurred in the inner part of Ariake Sea, Japan (5-14 September, 2012). This study aimed to gather information on the molecular mechanisms underlying the physiological responses in field C. marina population during HAB. Proteomic analysis showed that the abundance of -37{\%} protein spots (40 out of 108 detected from 2-DE gel images) significantly varied with the sampling date. Significant decreases in the abundances of proteins involved in photosystem II (LHCP 4), electron transfer chain (Cyt c553), Calvin cycle (GAPDH), and chloroplast antioxidant system (2-Cys Prx) were observed as the bloom progressed, suggesting the efficiencies of those photosynthetic pathways declined during the bloom. In addition, the abundances of the above proteins showed significant positive correlations with the Fv/Fm ratio and growth rate of C. marina and with DIN concentrations (except LHCP 4). Our findings suggested that declined expressions of those photosynthesis-related proteins presented some molecular foundation of the decreases in Fv/Fm ratio and growth rate of C. marina during the bloom, and also provided insight into mechanistic links between the external/internal factors and physiological responses of C. marina that may ultimately dictate the ecology of the bloom.",
author = "Xuchun Qiu and Kokl Mukai and Yohei Shimasaki and Michito Tsuyama and Tadashi Matsubara and Takashi Nakashima and Hirofumi Ichinose and Yu Nakajima and Ibuneo Honjo and Yuji Oshima",
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TY - JOUR

T1 - Variations in the expression of photosynthesis-related proteins in field Chattonella marina cells during a harmful algal bloom

AU - Qiu, Xuchun

AU - Mukai, Kokl

AU - Shimasaki, Yohei

AU - Tsuyama, Michito

AU - Matsubara, Tadashi

AU - Nakashima, Takashi

AU - Ichinose, Hirofumi

AU - Nakajima, Yu

AU - Honjo, Ibuneo

AU - Oshima, Yuji

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Time series variations in protein expression profiles in field Chattonella marina cells were investigated during a HAB occurred in the inner part of Ariake Sea, Japan (5-14 September, 2012). This study aimed to gather information on the molecular mechanisms underlying the physiological responses in field C. marina population during HAB. Proteomic analysis showed that the abundance of -37% protein spots (40 out of 108 detected from 2-DE gel images) significantly varied with the sampling date. Significant decreases in the abundances of proteins involved in photosystem II (LHCP 4), electron transfer chain (Cyt c553), Calvin cycle (GAPDH), and chloroplast antioxidant system (2-Cys Prx) were observed as the bloom progressed, suggesting the efficiencies of those photosynthetic pathways declined during the bloom. In addition, the abundances of the above proteins showed significant positive correlations with the Fv/Fm ratio and growth rate of C. marina and with DIN concentrations (except LHCP 4). Our findings suggested that declined expressions of those photosynthesis-related proteins presented some molecular foundation of the decreases in Fv/Fm ratio and growth rate of C. marina during the bloom, and also provided insight into mechanistic links between the external/internal factors and physiological responses of C. marina that may ultimately dictate the ecology of the bloom.

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