Variegated expression of a mouse steroid 21-hydroxylase/β-galactosidase transgene suggests centripetal migration of adrenocortical cells

Steven D. Morley, Isabelle Viard, Bon Chu Chung, Yayoi Ikeda, Keith L. Parker, John J. Mullins

Research output: Contribution to journalArticle

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Abstract

5'-Flanking sequences (6.4 kb) of the mouse steroid 21-hydroxylase (21- OHase) A gene linked to a LacZ reporter gene directed appropriate cell- specific expression in cultured Y1 adrenocortical tumor cells and in the adrenal cortex of transgenic mice. The transgene expression initiated at the same stage of adrenal development as the endogenous 21-OHase gene (embryonic day 11.5). Although the endogenous 21-OHase gene is expressed throughout the adrenal cortex, the 21-OHase/β-gal transgene showed a strikingly variegated pattern of adrenocortical expression in all 10 transgene-expressing mouse lines examined. This presents as radial stripes of β-gal staining transcending the classical zonal structure of the adrenal cortex but paralleling the columnar arrangement of cells of the zona fasciculata and the centripetal organization of the adrenocortical blood supply. To the extent that the variegated pattern of 21-OHase/β-gal transgene expression depicts adrenocortical cell lineage, these results suggest that all cells within an individual stripe have a common clonal origin; the radial pattern of clonally derived cells argues that cellular migration maintains the adult adrenocortical cell population. Adrenal glands of developing embryos also exhibited a variegated pattern of 21-OHase/β-gal transgene expression. However, this presented as islands of β-gal reporter staining within the developing gland, suggesting that the rapid embryonic adrenal growth phase, which precedes the establishment of the classic adrenocortical zonal structure, may be governed by cellular mechanisms distinct from those responsible for maintenance of the adult adrenocortical cell population.

Original languageEnglish
Pages (from-to)585-598
Number of pages14
JournalMolecular Endocrinology
Volume10
Issue number5
DOIs
Publication statusPublished - May 20 1996

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Galactosidases
Steroid 21-Hydroxylase
Transgenes
Cell Movement
Adrenal Cortex
Zona Fasciculata
Staining and Labeling
Genes
Lac Operon
5' Flanking Region
Cell Lineage
Adrenal Glands
Reporter Genes
Islands
Transgenic Mice
Population
Embryonic Structures
Maintenance
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

Cite this

Variegated expression of a mouse steroid 21-hydroxylase/β-galactosidase transgene suggests centripetal migration of adrenocortical cells. / Morley, Steven D.; Viard, Isabelle; Chung, Bon Chu; Ikeda, Yayoi; Parker, Keith L.; Mullins, John J.

In: Molecular Endocrinology, Vol. 10, No. 5, 20.05.1996, p. 585-598.

Research output: Contribution to journalArticle

Morley, Steven D. ; Viard, Isabelle ; Chung, Bon Chu ; Ikeda, Yayoi ; Parker, Keith L. ; Mullins, John J. / Variegated expression of a mouse steroid 21-hydroxylase/β-galactosidase transgene suggests centripetal migration of adrenocortical cells. In: Molecular Endocrinology. 1996 ; Vol. 10, No. 5. pp. 585-598.
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