TY - JOUR
T1 - Various spatiotemporal expression profiles of anther-expressed genes in rice
AU - Hobo, Tokunori
AU - Suwabe, Keita
AU - Aya, Koichiro
AU - Suzuki, Go
AU - Yano, Kentaro
AU - Ishimizu, Takeshi
AU - Fujita, Masahiro
AU - Kikuchi, Shunsuke
AU - Hamada, Kazuki
AU - Miyano, Masumi
AU - Fujioka, Tomoaki
AU - Kaneko, Fumi
AU - Kazama, Tomohiko
AU - Mizuta, Yoko
AU - Takahashi, Hirokazu
AU - Shiono, Katsuhiro
AU - Nakazono, Mikio
AU - Tsutsumi, Nobuhiro
AU - Nagamura, Yoshiaki
AU - Kurata, Nori
AU - Watanabe, Masao
AU - Matsuoka, Makoto
N1 - Funding Information:
The Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) Grants-in-Aid for Special Research on Priority Areas (Nos. 18075003 and 18075012 to M.W., 18075005 to N.T., 18075006 to M.M., 18075009 to N.K., 19043009 to T.I., 19043015 and 19651084 to K.Y.); the Japan Society for Promotion of Science (JSPS) Grants-in-Aid for the 21st Century Center of Excellence Program (to M.W.); the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF) grant for the Integrated Research Project for Plant, Insect, and Animal using Genome Technology (Nos. IPG-0003 to M.M., IPG-0012 to M.N., IPG-0019 to M.W.); a Research Fellowship for Postdoctoral Fellowships for Young Researchers of JSPS (to K.S.).
PY - 2008/10
Y1 - 2008/10
N2 - The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
AB - The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
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U2 - 10.1093/pcp/pcn128
DO - 10.1093/pcp/pcn128
M3 - Article
C2 - 18776202
AN - SCOPUS:54149084809
VL - 49
SP - 1417
EP - 1428
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 10
ER -