Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts

Tomoya Hayashi, Hisako Imamura, Koichi Nishiyama, Hirofumi Shintaku, Hidetoshi Kotera, Takashi Miura, Ryuji Yokokawa

Research output: Contribution to journalConference articlepeer-review

5 Citations (Scopus)

Abstract

In this paper, we present a poly-dimethylsiloxane (PDMS) microfluidic device to create a vascular network for a long-term spheroid culture, of which network and spheroid are consist of human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (LF), respectively. Following device design, fabrication, and fundamental evaluation of HUVEC sprouting conditions, we visualized that HUVEC networks were successfully formed by the co-culture with LFs and reached a LF-based spheroid. Moreover, perfusability of the network was evaluated by injecting fluorescent microbeads. This platform will be applicable for long-term tissue cultures to understand morphogenesis and modeling of blood vessel functions.

Original languageEnglish
Article number7050995
Pages (from-to)476-479
Number of pages4
JournalProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2015-February
Issue numberFebruary
DOIs
Publication statusPublished - Jan 1 2015
Event2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal
Duration: Jan 18 2015Jan 22 2015

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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