Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts

Tomoya Hayashi; Hisako Imamura; Koichi Nishiyama; Hirofumi Shintaku; Hidetoshi Kotera; Takashi Miura; Ryuji Yokokawa

doi:10.1109/MEMSYS.2015.7050995

Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts

Tomoya Hayashi, Hisako Imamura, Koichi Nishiyama, Hirofumi Shintaku, Hidetoshi Kotera, Takashi Miura, Ryuji Yokokawa

Bioregulation

Research output: Contribution to journal › Conference article › peer-review

6 Citations (Scopus)

Abstract

In this paper, we present a poly-dimethylsiloxane (PDMS) microfluidic device to create a vascular network for a long-term spheroid culture, of which network and spheroid are consist of human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (LF), respectively. Following device design, fabrication, and fundamental evaluation of HUVEC sprouting conditions, we visualized that HUVEC networks were successfully formed by the co-culture with LFs and reached a LF-based spheroid. Moreover, perfusability of the network was evaluated by injecting fluorescent microbeads. This platform will be applicable for long-term tissue cultures to understand morphogenesis and modeling of blood vessel functions.

Original language	English
Article number	7050995
Pages (from-to)	476-479
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2015-February
Issue number	February
DOIs	https://doi.org/10.1109/MEMSYS.2015.7050995
Publication status	Published - Jan 1 2015
Event	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, Portugal Duration: Jan 18 2015 → Jan 22 2015

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2015.7050995

Cite this

Hayashi, T., Imamura, H., Nishiyama, K., Shintaku, H., Kotera, H., Miura, T., & Yokokawa, R. (2015). Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2015-February(February), 476-479. [7050995]. https://doi.org/10.1109/MEMSYS.2015.7050995

Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts. / Hayashi, Tomoya; Imamura, Hisako; Nishiyama, Koichi et al.

In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Vol. 2015-February, No. February, 7050995, 01.01.2015, p. 476-479.

Research output: Contribution to journal › Conference article › peer-review

Hayashi, T, Imamura, H, Nishiyama, K, Shintaku, H, Kotera, H, Miura, T & Yokokawa, R 2015, 'Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts', Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2015-February, no. February, 7050995, pp. 476-479. https://doi.org/10.1109/MEMSYS.2015.7050995

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AU - Kotera, Hidetoshi

AU - Miura, Takashi

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Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts

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