Vasopressin-induced contraction of uterus is mediated solely by the oxytocin receptor in mice, but not in humans

Masaki Kawamata, Minori Mitsui-Saito, Tadashi Kimura, Yuki Takayanagi, Teruyuki Yanagisawa, Katsuhiko Nishimori

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In the non-pregnant mouse myometrium, both arginine vasopressin and oxytocin induced contractions (pD2=8.55±0.13 and 9.23±0.09, respectively). The effect of oxytocin was the most potent, while the maximum contractions induced by these two peptides were almost of the same magnitude. Both vasopressin- and oxytocin-induced contractions were strongly inhibited by an oxytocin receptor antagonist, CL-12-42 (d(CH 2)5[Tyr(Me)2,Thr4,Tyr-NH 2 9]OVT), and weakly inhibited by a vasopressin V 1a receptor antagonist, SR49059 ((2S)1-[(2R,3S)-5-chloro-3-(2-chlorophenyl)-1-(3,4-dimethoxybenzene-sulfonyl) -3-hydroxy-2,3-dihydro-1H-indole-2-carbonyl]-pyrrolidine-2-carboxamide). Similar results were obtained in the pregnant mouse myometrium. These results suggest that not only oxytocin- but also vasopressin-induced contraction is mediated by the activation of oxytocin receptors in the mouse myometrium. A reverse transcription polymerase chain reaction study failed to reveal mRNA of the vasopressin V1a receptor in the mouse myometrium. In contrast, in the non-pregnant human myometrium, vasopressin-induced contraction was inhibited by SR49059. Oxytocin showed no effect on the myometrium. These results suggest that there are significant differences in the functional receptors and contractile responses to vasopressin and oxytocin in the human and mouse uteri.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalEuropean Journal of Pharmacology
Volume472
Issue number3
DOIs
Publication statusPublished - Jul 11 2003
Externally publishedYes

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Myometrium
Vasopressins
Uterus
Oxytocin
Oxytocin Receptors
Vasotocin
Vasopressin Receptors
Arginine Vasopressin
Reverse Transcription
mouse OXTR protein
Polymerase Chain Reaction
Messenger RNA
Peptides

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Vasopressin-induced contraction of uterus is mediated solely by the oxytocin receptor in mice, but not in humans. / Kawamata, Masaki; Mitsui-Saito, Minori; Kimura, Tadashi; Takayanagi, Yuki; Yanagisawa, Teruyuki; Nishimori, Katsuhiko.

In: European Journal of Pharmacology, Vol. 472, No. 3, 11.07.2003, p. 229-234.

Research output: Contribution to journalArticle

Kawamata, Masaki ; Mitsui-Saito, Minori ; Kimura, Tadashi ; Takayanagi, Yuki ; Yanagisawa, Teruyuki ; Nishimori, Katsuhiko. / Vasopressin-induced contraction of uterus is mediated solely by the oxytocin receptor in mice, but not in humans. In: European Journal of Pharmacology. 2003 ; Vol. 472, No. 3. pp. 229-234.
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